Question

You cut a gene of interest with HincII and would like to insert it into the multiple cloning site (MCS) of a plasmid. The plasmid's MCS contains the cut sites listed below.

Which of these sites could you use to insert the gene of interest? Select all that apply.

GTC*GAC 1.
CAG*CTG

G*TYRAC 2.
CARYT*G

GT*CGAC 3.
CAGC*TG

GGT*ACC 4.
CCA*TGG

GTA*TAC 5.
CAT*ATG

none of these sites

Answer 1

1 and 5

Hind 11 site : 5' GT YTRAC z 3 CARA YTG 51 1)s G-TCGAC 3' 3' CAP 795 Y=C,T R=A,G 2)5'GTA ·TAC3 3 CARJ Yaj TG5'

Orange colour denotes the sequence in the option given.

Restriction enzymes are the enzymes which cut DNA at a specific location. They are also called as molecular scissors. They are widely used in recombinant DNA technology experiments. If we want to isolate a gene from the DNA of an organism, we cut the gene using specific restriction enzyme. And then the gene is inserted into a specific vector which is either cut with the same restriction enzyme which was used to cut the gene or it it is cut with an enzyme which will give compatible ends that can ligate with the cut ends of the genes.

This is the principle of this question. You have to check all the options one by one which are forming compatible ends with the the site of HindII.

Please rate high.