8. Bgl II:
A^GATCT
TCTAG^A
Xho I:
C^TCGAG
GAGCT^C
Resistance marker on the vector = Ampicillin
1. No colonies on plate with bacteria with no vector
2. Since BglII and XhoI produce incompatible sticky ends, they
cannot ligate together to produce self ligated vector.
So, the number of colonies on the plate will be almost zero
(Assuming 100% restriction digestion efficiency)
3. Highest number of colonies can be observed
4. No colonies on plate with no bacteria
i need help with the whole page! 8. You try to ligate a cDNA fragment with...
Cloning 2 Below is the restriction map of a 10 kb piece of DNA. Also shown below is a cloning vector which has two unique restriction enzyme recognition sites, one for EcoRI (E) and one for HindIII (H). The location of the kanamycin (kan) and ampicillin (amp) resistance genes is also shown. Kanamycin and ampicillin are antibiotics that are commonly used to select transformed E. colicells (consult the Lab Manual for more information). Note that the HindIII site is located...
1. Fill in the table above with what you observe on your plates. 2. Bacterial transformation occurred on which agar plate (s)? What evidence do you have that the bacteria were transformed here? 3. Which plates have glowing growth? Explain what causes bacteria to glow. II. Transformation of E. coli with Plasmid DNA (PGLO) 1. Three LB (Luria Broth) agar plates are obtained. The plates contain: • Plate A: LB-Agar/Ampicillin/Arabinose • Plate B: LB-Agar/Ampicillin • Plate C: LB-Agar 2. Three...
Need help filling in the chart and answering the questions that go along with it. I have added the procedure and the instructions as well as the "results" that are supposed to be used to fill in the chart. Thank you! We were unable to transcribe this imageTABLE 8-1 Cast of Characters and a Legend of Abbreviations Name Symbol Function in This Experiment Green fluorescent protein GFP It serves as an indicator of successful transformation and gene transcription expression in...
microbiology help TOT Zoo Add Page Insert Table Chart Text Shape Media Comment These questions will serve in lieu of a lab report for Exercise 15, 16, and 17 You will find the answer to these questions in the background, procedure, results and interpretation sections of manual Exercise 15, 16, and 17, videos, Actions of Selective and Differential Media Chart, and the Principle/Theory article in homework section.) General Questions 1. What is the purpose (function) of selective media? (How does...