The equation that describes the above Michaelis-Menten curve: Vo TS]+K Vmax [S] Michaelis-Menten Equation Lineweaver and Burke manipulated the Michaelis-Menten equation to yield: Ko V I S Vmax [S] Lineweaver-Burke Equation Linewenver Burke Equation If you plot 1/ V. vs. 1/[S], you get the following Lineweaver-Burke plot: 1/V. Slope = km/Vmax Intercept = -1/KM -Intercept = 1/Vmax 1/[S] Which is easier to calculate values for Km and Vmax, using the linear (y=mx+b) Lineweaver-Burke Plot or the Michaelis-Menten curve?
(I need help with part C, Drawing the expected Michaelis-Menten plot; Do NOT draw the Lineweaver-Burk plot. thanks!) 1. Michaelis-Menten kinetics- use the M-M equation to answer the following: a. An enzyme (5 µM) has a Vmax of 450 mM/min. What is kcat? b. When the substrate concentration is 50 mM, the initial velocity (V0) was measured to be 375 mM/min. Under the conditions described above, calculate the KM. c. Draw the expected Michaelis-Menten plot (label your axes and include...
Use the Michaelis-Menten Plot to answer question 11. If V max for the black curve is 1, what is Km? Which graph (A, B, C, or D) represent a competitive inhibition? Determine Km for your answer in b. How does competitive inhibition affect Vmax and Km?
The Eadie-Hofstee plot shown below, is an alternative graphical representation of Michaelis-Menten kinetics. t plots the rate (v) versus the ratio of the rate over the substrate concentration (vIS]). This plot is typically used to determine the maximum rate, Vmax, and the Michaelis constant, Km, which can be gleaned from the intercepts and slope. Identify each intercept and the slope in terms of the constants Vmax and Km Eadie-Hofstee Plot y-intercept Slope x-intercept AS
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5.How would you determine the values of KM and Vmax from a: a) Michaelis-Menten plot b) Lineweaver-Burke plot 6. How is general acid/base catalysis different from regular acid/base catalysis? 7. How would you recognize the process of covalent catalysis in an enzyme reaction mechanism?
what are the advantages and disadvantages of a Dot Plot graph?
1. Using Excel, make a Michaelis-Menten plot from the following data. Properly label the axes with units. Calculate the Km and Vmax from the data and compare your answer with the graph. Substrate Concentration Velocity (umol/min) (mm) 2.500 0.588 1.000 0.500 0.714 0.417 0.526 0.370 0.250 0.256
4. Basic concepts of Michaelis-Menten kinetics. The Michaelis-Menten equation is expression of the relationship between the initial velocity, Vo, of an enzymatic reaction and substrate concentration, [S]. There are three conditions that are useful for simplifying the Michaelis-Menten equation: [S] <<Km; [S] = Km; [S] >> Km. Match each condition with the statement(s) that describe it. TV, Vmox[S] Vo =Vmax m . V Vo - Vmax [S] Km +[S] V. (um/min) max [S] (mm) (a) Doubling [S] will almost double...
6. The Michaelis-Menten curve for enzyme X is shown below for substrate A. Indicate where on the plot you would determine Kcat, Km and kca/Km- (1 points) 400 300 F 200F 100 500 1000 1500 2000 substrate A, HM 6. The Michaelis-Menten curve for enzyme X is shown below for substrate A. Indicate where on the plot you would determine Kcat, Km and kca/Km- (1 points) 400 300 F 200F 100 500 1000 1500 2000 substrate A, HM