using my standard curve I need to determine the concentration of glucose in the unknown the...
using my standard curve I need to determine the concentration of glucose in the unknown the equation is y=-0.1741x+0.7254 the absorbance for the unknown is 0.201
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using my standard curve I need to determine the concentration of
glucose in the unknown the...
You have just created a standard curve of glucose concentration as a function of ANS absorbance...
You have just created a standard curve of glucose concentration as a function of ANS absorbance values. Using the equation of a line (y= 1.0357x), what absorbency is associated with a glucose concentration of of 0.71? y = 1.0357x R2 = 0.9843 Absorbance (540nm) 0 0.2 1 1.2 0.4 0.6 0.8 Glucose Concentration (mg/mL)
What is the reason behind developing a standard curve to determine concentration in an unknown solution?...
What is the reason behind developing a standard curve to
determine concentration in an unknown solution?
What is the reason behind developing a standard curve to determine concentration in an unknown solution? 5.
need help with Concentration solutions Determine the Cu?molar concentration of each unknown solution. For unknown Cu?...
need help with Concentration solutions
Determine the Cu?molar concentration of each unknown solution. For unknown Cu? solution 1, the absorbance = 0.176 at 675 nm. Unknown Cul solution 1 concentration = _м For unknown Cu? solution 2, the absorbance =0.827 at 675 nm. Unknown Cu? solution 2 concentration - M Logger Profile Edit Experiment Data Analyze Insert Options Page Help Help Untitled AoQ 121/ O - Page 1 - No Device Connected Data Set ConcAbs (M 0.012 0.055 0.024 0.106...
2. The AMR for a method for measuring glucose has been determined by the manufacturer to...
2. The AMR for a method for measuring glucose has been determined by the manufacturer to be 20-500 mg/dL A. The data for its verification study is shown below. Is the method's linearity verified for this range? Standard Standard [Glucose] mg/dL Absorbance Blank Standard 1 Standard 2 Standard 3 Standard 4 Standard 5 Standard 6500 0.000 0.050 0.099 0.201 0.298 0.402 0.501 50 100 200 300 400 B. The method allows a maximum dilution by a factor of 10. What...
They performed a BSA assay to determine the concentration of an unknown solution X. The graph...
They performed a BSA assay to determine the concentration of an unknown solution X. The graph below is the BSA standard curve. In the cuvette, you add 25 ul of the unknown solution X, 200 ul of the Bradford’s reagent and 775 ul dH2O. You obtain an absorbance of 0.58 at 595nm. Use all the information to calculate the concentration of the unknown solution X. Show work Y- 0.0457x R^2- 0.9901
2. A linear standard curve of KMnO is prepared from a set of standard solutions by...
2. A linear standard curve of KMnO is prepared from a set of standard solutions by plotting absorbance (y) vs. concentration (x). Use Logger Pro (as described in the Data Analysis for this experiment), or Google Sheets/Excel, to plot this standard curve using the data in Table 1. Help with spreadsheets [PDF] Table 1: Data for the standard curve of KMnO4 Absorbance Concentration (M) 0.330 6.00 x 10-4 0.264 4.80 x 10-4 0.198 3.60 x 10-4 0.132 2.40 x 10-4...
A Bradford assay was carried out to measure protein concentration. The absorbance of five standard samples...
A Bradford assay was carried out to measure protein concentration. The absorbance of five standard samples and two unknown samples are listed in the table below. The Bradford reaction mixture (1 ml) was prepared as follows: 100 μl sample A or sample B + 200 μl Bradford reagent + 700μl 0.9% NaCl solution sample ID concentration (mM) absorbance (O.D.) std 1 100 0.78 std 2 50 0.4 std 3 25 0.18 std 4 12.5 0.08 std 5 0 0 ...
1. Use the following data to construct a calibration curve and determine the concentration of unknown...
1. Use the following data to construct a calibration curve and determine the concentration of unknown as given. What is the molar absorptivity of the analyte? The compound solutions were measured in a 0.5 cm path length quartz cuvette. solution [X] (M) Absorbance At 254 nm Standard 1 Standard 2 Standard 3 Standard 4 Standard 5 Sample 0.03584 0.05719 0.1289 0.2305 0.3161 ??? 0.097 0.169 0.365 0.682 0.997 0.719
please help!! will give feedback! Examine the given Beer's law standard curve for an unknown dye...
please help!! will give feedback!
Examine the given Beer's law standard curve for an unknown dye measured in a 1.0 cm cuvette. Absorbance of Unknown Dye at 542 nm Absorbance at 542 nm y = 15200x -0.018 5.00E-06 1.00E-OS 1.50E-05 2.00E-05 2.50E-053.006-053.50E-OS Concentration (M) What is the concentration (in M) of a sample of the unknown dye with an absorbance of 0.25 at 542 nm? Answer:
determine the concentration of glucose in the original glucose solution. report a relative standard deviation for...
determine the concentration of glucose in the original glucose solution. report a relative standard deviation for the original concentration Glucose solution / mL: Tube 1: 0 mL Tube 2: 2 mL Tube 3: 4 mL Tube 4: 6 mL Tube 5: 8 mL Tube 6: 9.5 mL Absorbance at 420 nm for each solution: Tube 1: 0.617 Tube 2: 0.578 Tube 3: 0.736 Tube 4: 0.653 Tube 5: 0.843 Tube 6: 0.895
You have just created a standard curve of glucose concentration as a function of ANS absorbance values. Using the equation of a line (y= 1.0357x), what absorbency is associated with a glucose concentration of of 0.71? y = 1.0357x R2 = 0.9843 Absorbance (540nm) 0 0.2 1 1.2 0.4 0.6 0.8 Glucose Concentration (mg/mL)
What is the reason behind developing a standard curve to
determine concentration in an unknown solution?
What is the reason behind developing a standard curve to determine concentration in an unknown solution? 5.
need help with Concentration solutions
Determine the Cu?molar concentration of each unknown solution. For unknown Cu? solution 1, the absorbance = 0.176 at 675 nm. Unknown Cul solution 1 concentration = _м For unknown Cu? solution 2, the absorbance =0.827 at 675 nm. Unknown Cu? solution 2 concentration - M Logger Profile Edit Experiment Data Analyze Insert Options Page Help Help Untitled AoQ 121/ O - Page 1 - No Device Connected Data Set ConcAbs (M 0.012 0.055 0.024 0.106...
2. The AMR for a method for measuring glucose has been determined by the manufacturer to be 20-500 mg/dL A. The data for its verification study is shown below. Is the method's linearity verified for this range? Standard Standard [Glucose] mg/dL Absorbance Blank Standard 1 Standard 2 Standard 3 Standard 4 Standard 5 Standard 6500 0.000 0.050 0.099 0.201 0.298 0.402 0.501 50 100 200 300 400 B. The method allows a maximum dilution by a factor of 10. What...
2. A linear standard curve of KMnO is prepared from a set of standard solutions by plotting absorbance (y) vs. concentration (x). Use Logger Pro (as described in the Data Analysis for this experiment), or Google Sheets/Excel, to plot this standard curve using the data in Table 1. Help with spreadsheets [PDF] Table 1: Data for the standard curve of KMnO4 Absorbance Concentration (M) 0.330 6.00 x 10-4 0.264 4.80 x 10-4 0.198 3.60 x 10-4 0.132 2.40 x 10-4...
1. Use the following data to construct a calibration curve and determine the concentration of unknown as given. What is the molar absorptivity of the analyte? The compound solutions were measured in a 0.5 cm path length quartz cuvette. solution [X] (M) Absorbance At 254 nm Standard 1 Standard 2 Standard 3 Standard 4 Standard 5 Sample 0.03584 0.05719 0.1289 0.2305 0.3161 ??? 0.097 0.169 0.365 0.682 0.997 0.719
please help!! will give feedback!
Examine the given Beer's law standard curve for an unknown dye measured in a 1.0 cm cuvette. Absorbance of Unknown Dye at 542 nm Absorbance at 542 nm y = 15200x -0.018 5.00E-06 1.00E-OS 1.50E-05 2.00E-05 2.50E-053.006-053.50E-OS Concentration (M) What is the concentration (in M) of a sample of the unknown dye with an absorbance of 0.25 at 542 nm? Answer:
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