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They performed a BSA assay to determine the concentration of an unknown solution X. The graph...

They performed a BSA assay to determine the concentration of an unknown solution X. The graph below is the BSA standard curve. In the cuvette, you add 25 ul of the unknown solution X, 200 ul of the Bradford’s reagent and 775 ul dH2O. You obtain an absorbance of 0.58 at 595nm. Use all the information to calculate the concentration of the unknown solution X. Show work  

Y- 0.0457x

R^2- 0.9901

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Answer #1

Let -- - the concentralin of unknown solution = 2 volume of unknown solution = 25 ML -Absorbance =0.58 at x = 595 nm. Given Y

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