Question

In a chromatography experiment, how do you identify the components of an unknown mixture?

Select one:

Combine the mixture with multiple known compounds and look for the largest spots.

Compare the R_f values of the mixture components to the R_f values of pure known compounds.

Combine the mixture with a series of known compounds and observe the color of the dots.

Compare the color of the mixture spots to the colors of pure known compounds.

Answer 1

- R (Retention factor) is defined as ratio of distanced travelled by component & distance travelled by sduent front - - distance travelled by schvent distance travelled by solvent front Suppose There are 3 components of mixture which we have to seperate e they are ca ennen with the like this que LE aby AVE

The silica gel Tic of compounds A, B, C A solvent front- Laul 1.8m 1.sun o. Sunt I Gesign A B .. - R; (B) = 7 All Rf6A) = 004 these 3 2 components travel 115 = 0.75 2008 at different rate. -Rf() = 12 = 0.9 Hence, all three components of mixture A,B,C have different f values On comparing af values of the mixture components to Rj values of pure - known compound Components of Guit nous mixture can be identified: