Question

13. Explain this statement: An Enzyme follows Michaelis-Menten kinetics, meaning explain the characteristics of a M-Menzyme and the type of mechanism it uses. Also, include definitions of Km and Vmax. (This is that synthesis question.) 14. Compare and contrast E-S interactions with P-L interactions.

Answer 1

Mechanism 5+ 6 ] [SE] wypt E o the The enzyme first combine with the substrate to forum [15] Complex, which em with the free is in equ W id enzyme and finally this complex is transton product with the regeneration of enzyme that he an [E] and [57 are the initial cone Substate and enzyme and finally this into product with the regeneratia let, enzyme and substrate respectively, Indes oma Cone of free enzyme tue fos ara host 90 sheehv clearly, [E] = LE) - (SE] Kifo cha poligM role as soos enzyme ? Come of free substrate, [s] = [50] - [SE] . Tonband ghetto sto (153 [soas [].» [E]. . . » [SE] According to Michaelis - Menten [SE] is a van Holl Complex, hence we use Steady state approx. Auk d[3] . Kils][€] - Ky [se] – kg. [38] 20 - W. [s] [E] - [se}} - K, LE) - ky[se] => Singe (SE= K, [s(t)o bo oh at 4.410fast sa pred obrobook was Il faut ut, og eit K24 K, [s] E] [5] D S 37 Kitky + 5] - KM + [6] 1 Km = K tk . KL SERVER

KM is called the Michal is constant in Rete of the reaction, =Kq [SE] We a kq [E]. [s] Kyat [57 P Michaelis - Menten egun s low, is ineler when substate Cone [s] << KM, ne = kg [E]. s] in this case, the reaction is 1st oder warto subshate and the overall anda = 2, 1st oder writo substate and list ander wn to enzyme un alt When, substrate cone, ů very high, [s] » Koyo v=K CE] Imax af vo in this case, the rin i zno order wr to Subshate ! Hom the Onerall oder gs 1 Uman is the maximum veelocity of the reaction, 4 = Nomex angiero is 1 der 00 If there is only one achine site per enzyme mobant, then kq 21 Iman representa no of Subshate molecule Mansformed into pridact persee per active site of the enzym which is called the turn oner frequency, to ... Then, Michalys - Mentin egun reduces to N = Imax [s ] . s svars KM + [5] mt ry

Uman is maximum rate of the reaction. man is achieved when rå [s] » Km Km į the M-M constant. . we knowd; v= Uman. [s] Km + [5] at Wa Wanyone So, Vimean . U'mon [s] en So, an Viorent RM + [5] 12 4 Km + [s] - 2[5] a Kve = [s]. . So, at walmar, the Substrate Concentration i s equal to Km. ma 2 en m