DNA | mRNA | amino acid | mutation | effect | |
wild type |
3'CTT5' 5'GAA3' |
5'GAA3' | glu | N/A | wild type |
mutant 1 |
3'CAT5' 5'GTA3' |
5' GUA3' | val | subsitution (transversion ) | mutant |
mutant 2 |
3'CTC5' 5'GAG3' |
5'GAG3' | glu | subsitution (transition ) | mutant |
mutant 3 |
3'ATT5' 5'TAA3' |
5'UAA3' | stop codon | subsitution (transversion ) | mutant |
subsitution mutation is of two types:
transversion : when purine is replaced by pyrimidine or pyrimidine is replaced by purine
transition : when purine is replaced by purine or pyrimidine is replaced by pyrimidine .
purines : A,G
pyrimidine : C,T
Complete the table below. [6] mRNA Amino Acid Mutation Effect Wild-type 5-GAA-3 Glu N/A Wild-type Mutant...
mRNA transcr leaves the mucl ke protcins Th eings the amino no acids anre the bui ead in onder to. start and stop mak Land when to stot C. Use your codon chart to determine the amino acid sequence. Remember to read through the strand and ONLY start on AUG and STOP when it tells you to stop Follow example below Example: DNA AGA CGG TAC CTC CGG TGG GTG CTT GTC TGT ATC CTT CTC AGT ATC UCU GCC...
Type of DNA/RNA mutation: Type of protein mutation: 2. TTT 5' ATG 3" TAC GAG cтa GCT cGA CTT GAA GAA cтт AAA AAA ттт TAA ATT 3' 5 The corresponding mRNA would then be: And the protein that would be formed would be: Type of DNA/RNA mutation: Type of protein mutation:
please help with #4 and #5 question 5 of 5 the cross lines indicate deletion of the nucleotides. what type of mutation is this? what is the resulting polypeptide sequence? 3'- GTT - TAC - CTT - -CTC--TCC -TCA -GAC - ATC -GCA -5' (template strand) 5' - CAA - ATG - GAA - -GAG--AGG - AGT - CTG- TAG - CGT - 3' (non-template/coding strand) answer: It's a ___________________mutation. Final polypeptide sequence is N' - Met - GLU _______________ ...
Example question (p. 171). The amino acid sequence of a wild-type protein is Met-His-Ala-Trp-Asn-Gly-Glu–His-Arg The amino acid sequences of two mutants are: Mutant 1: Met-His-Ala-Trp-Lys-Gly-Glu–His-Arg Mutant 2: Met-His-Ala For each mutant, specify the type of mutation that has occurred, using the mutation classification system based on effect to protein function
The wild-type sequence of part of a protein is Trp-Trp-Trp-Met-Arg-Glu-Trp-Thr-Met Each mutant in the following table differs from wildtype by a single point mutation. Using this information, determine the mRNA sequence coding for the wild-type polypeptide. If there is more than one possible nucleotide, all list possibilities. Mutant Amino Acid Sequence of Polypeptide Trp-Trp-Trp Met Trp-Trp-Trp-Met-Arg-Asp-Trp-Thr-Met Trp-Trp-Trp-Met-Arg-Lys-Trp-Thr-Met Trp-Trp-Trp-Met-Arg-Glu-Trp-Met-Met The wild-type sequence of part of a protein is Trp-Trp-Trp-Met-Arg-Glu-Trp-Thr-Met Each mutant in the following table differs from wildtype by a single...
Shown below are the amino acid sequences of the wild-type and three mutant forms of a short protein. Each mutation results from a single nucleotide change (transition/transversion / insertion / deletion). Use this information to answer the following questions. Hint: First, reconstruct as much as you can of the wild-type RNA sequence and then reference that sequence when analyzing the mutations. Wild type: met - gin-ala - ser-val - arg - phe Mutant 1: met - gln - pro-ser -...
Shown below are the amino acid sequences of the wild-type and three mutant forms of a short protein. Each mutation results from a single nucleotide change (transition / transversion / insertion / deletion). Use this information to answer the following questions. Hint: First, reconstruct as much as you can of the wild-type RNA sequence and then reference that sequence when analyzing the mutations. Wild type: met – gln – ala – ser – val – arg – phe Mutant 1:...
The table shows the partial sequences of a wild type polypeptide and three mutant polypeptides as well as the type of single nucleotide mutation that produced each mutant polypeptide. Peptide sequence Type of mutation Wild type Met - Leu - Arg - Ile - ... Mutant 1 Met - Leu - Arg - Met -... transition Mutant 2 Met - Leu - [STOP] transition Mutant 3 Met - Phe - Arg - lle - ... transversion Determine the mRNA sequence...
Below are several DNA sequences that are mutated compared with the wild-type sequence: 3-TAC TGACTGACGAT C-5. Envision that each is a section of a DNA molecule that has separated in preparation for transcription, so you are only seeing the template strand. Construct the complementary DNA sequences (indicating 5' and 3' ends) for each mutated DNA sequence, then transcribe (indicating 5' and 3' ends) the template strands, and translate the mRNA molecules using the genetic code, recording the resulting amino acid...
Below is the coding DNA sequence for a gene called tribble, including the wild-type and several mutant alleles. For each mutant allele, fully classify the mutation and its effect in the amino acid sequence. Wild-type 5'-ATGGCAACTACATATAGCACAGTTTGAACC-3' a. Mutant allele #1 5'-ATGGCAACTACATAAAGCACAGTTTGAACC-3' b. Mutant allele #2 5'-ATGGCAACTACATATAGCATAGTTTGAACC-3' c. Mutant allele #3 5'-ATGGCAACTACATACAGCACAGTTTGAACC-3' d. Which mutant allele is most likely to be a knockout and why?