Homework Answers
Answer 20 :- protoplast fusion
The fusion with protoplast is a physical phenomenon. Two or more protoplasts come into contact during fusion and adhere to each other either spontaneously or in the presence of chemicals which induce fusion.
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Question 20 Which does not add a specific gene to a cell? O protoplast fusion gene...
Question 19 Restriction enzymes are naturally produced by and isolated from O bacteria. O bacteriophages. O...
Question 19 Restriction enzymes are naturally produced by and isolated from O bacteria. O bacteriophages. O human cells. O plant cells. non-human mammals. Question 20 Which does not add a specific gene to a cell? O protoplast fusion O gene gun O microinjection O electroporation O transformation Question 21 Restriction endonuclease sites are often purposely located in vectors bacterial genomes 0 eukaryotic genomes therapeutic applications the polymerase chain reaction
Foreign DNA is acquired and expressed by a cell through taking in naked DNA. This is...
Foreign DNA is acquired and expressed by a cell through taking in naked DNA. This is a horizontal transfer and transformation b vertical transfer and transformation c horizontal transfer and transduction d expressive transfer and translocation e horizontal transfer and transfection Replica plating is used a to identify an auxotrophic mutant b to identify heterotrophic mutants c for cell viability counts d to preserve a partciular culture e to show selective growth on media H how is an RFLP different...
Which of the following can be used to transfer a vector into a host cell? O...
Which of the following can be used to transfer a vector into a host cell? O A. Gene gun OB. Microinjection OC. Transformation OD. all of the above Reset Selection
Chimeric gene-fusion products can be used for medical or industrial purposes. One idea is to produce...
Chimeric gene-fusion products can be used for medical or industrial purposes. One idea is to produce biological therapeutics for human medical use in animals from which the products can be easily harvested−in the milk of sheep or cattle, for example. The transgene construct will include: Select the two sequences that apply. -regulatory sequences of sheep insulin -sequences of a specific promoter and terminator -sequences that direct proper cell-type-specific expression -sequences that direct secretion of insulin -sequences of secretory molecules of...
please provide an explanation and/or a source if available. Thanks QUESTION 4 2 points Which one...
please provide an explanation and/or a source if available.
Thanks
QUESTION 4 2 points Which one of the following cell wall components does NOT use exocytosis to get out of the protoplast into the cell wall? Cellulose Hemicelluloses Expansins Pectins
QUESTION 1 Imagine you are preparing an industrial enzyme assay. The protocol says to add 20...
QUESTION 1 Imagine you are preparing an industrial enzyme assay. The protocol says to add 20 mL of 100 mM NaCl to your assay, and the final volume of the assay is 100 ml. What is the working concentration of NaCl? The number of significant digits you include in your answer doesn't matter, but it must be in units of mM. QUESTION 2 Imagine you are preparing an enzyme assay that requires a working concentration of 10 mM NaCl. If...
Which aspect of cell biology is NOT involved in the regulation of gene expression? Chromatin remodeling...
Which aspect of cell biology is NOT involved in the regulation of gene expression? Chromatin remodeling The make-up of a promoter Alternative splicing The overall size of RNA polymerase Presence of specific transcription factors
Question 8 0.5 pts Which of the following statements is false as it pertains to gene...
Question 8 0.5 pts Which of the following statements is false as it pertains to gene expression? O External cues have no impact on gene expression. O Specialized cells in a multi-cellular organism are capable of altering their patterns of gene expression in response to extracellular cues. O Different cell types can respond in different ways to the same extracellular signal. o Cortisol signals liver cells to boost the production of glucose. o Cortisol signals induce fat cells to reduce...
Question 3 3 pts What makes GFP fusion proteins more useful than western blotting when studying...
Question 3 3 pts What makes GFP fusion proteins more useful than western blotting when studying expression of a specific gene during development? Select all correct. Western blotting requires you to kill the cells, but GFP fusions let you see the protein in living cells Western blotting lacks the spatial and temporal information that you can get GFP fusion proteins Western blotting detects mRNA, not protein. Western blotting amplifies the amount of total protein present, which prevents you from understanding...
gene transfer like conjugatione 5. What is calcium chloride transformation? What does it accomplish? Calcium Chloride...
gene transfer like conjugatione 5. What is calcium chloride transformation? What does it accomplish? Calcium Chloride trensformation increases the ability of a bacteria call to in corporale plasmid DNA which callows it to be genetically frensformed. If accomplishes binding op plasmid DNA IV. 6. Why would you expect to find cell growth on the Luria nutrient agar plate? (LB plate)? 7. Did you expect to find cell growth on the ampicillin Luria plate? Explain WHY or WHY NOT. (LB/amp plate...
Question 19 Restriction enzymes are naturally produced by and isolated from O bacteria. O bacteriophages. O human cells. O plant cells. non-human mammals. Question 20 Which does not add a specific gene to a cell? O protoplast fusion O gene gun O microinjection O electroporation O transformation Question 21 Restriction endonuclease sites are often purposely located in vectors bacterial genomes 0 eukaryotic genomes therapeutic applications the polymerase chain reaction
Which of the following can be used to transfer a vector into a host cell? O A. Gene gun OB. Microinjection OC. Transformation OD. all of the above Reset Selection
please provide an explanation and/or a source if available.
Thanks
QUESTION 4 2 points Which one of the following cell wall components does NOT use exocytosis to get out of the protoplast into the cell wall? Cellulose Hemicelluloses Expansins Pectins
QUESTION 1 Imagine you are preparing an industrial enzyme assay. The protocol says to add 20 mL of 100 mM NaCl to your assay, and the final volume of the assay is 100 ml. What is the working concentration of NaCl? The number of significant digits you include in your answer doesn't matter, but it must be in units of mM. QUESTION 2 Imagine you are preparing an enzyme assay that requires a working concentration of 10 mM NaCl. If...
Question 8 0.5 pts Which of the following statements is false as it pertains to gene expression? O External cues have no impact on gene expression. O Specialized cells in a multi-cellular organism are capable of altering their patterns of gene expression in response to extracellular cues. O Different cell types can respond in different ways to the same extracellular signal. o Cortisol signals liver cells to boost the production of glucose. o Cortisol signals induce fat cells to reduce...
Question 3 3 pts What makes GFP fusion proteins more useful than western blotting when studying expression of a specific gene during development? Select all correct. Western blotting requires you to kill the cells, but GFP fusions let you see the protein in living cells Western blotting lacks the spatial and temporal information that you can get GFP fusion proteins Western blotting detects mRNA, not protein. Western blotting amplifies the amount of total protein present, which prevents you from understanding...
gene transfer like conjugatione 5. What is calcium chloride transformation? What does it accomplish? Calcium Chloride trensformation increases the ability of a bacteria call to in corporale plasmid DNA which callows it to be genetically frensformed. If accomplishes binding op plasmid DNA IV. 6. Why would you expect to find cell growth on the Luria nutrient agar plate? (LB plate)? 7. Did you expect to find cell growth on the ampicillin Luria plate? Explain WHY or WHY NOT. (LB/amp plate...
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