Question

Exercise # 1
A 60-year-old woman with anemia is admitted to the hospital. Her hematocrit is 17% and she has been experiencing subtle gastrointestinal bleeding over many weeks. Her physician requests 4 units of red blood cells for transfusion. The patient’s RBCs phenotyped as group AB, D-positive. Her antibody screen is negative on the sample drawn in the emergency room, but her records indicate a previously detected anti-E. Only three group AB, D-positive red blood cell units are available in the blood bank’s inventory. The blood bank’s inventory contains RBC donor units of all ABO and D types. Having located 6 E-negative donor units, you perform crossmatching on the units. One of the units is incompatible in the antiglobulin phase (2+). The physician is becoming insistent on beginning the transfusion since the patient is having some shortness of breath.
6. List several reasons to explain the one incompatible donor unit.

7. What additional testing do you perform?

Antibody-identification testing reveals no detectable antibodies, and the autologous control is also negative. Further testing revealed a positive DAT on the incompatible donor unit.
8. What is the usual transfusion service policy on donor units with a positive DAT?

Answer 1

The reasons for the incompatibility of donor unit includes presence of alloantibody,clerical error,gender (female more affected),other disease coditiond(like thalassemea),AIHA, malignancy

7.The tests include indirect coombs test,