the amount of strep in the plates is 100 ug/ml. if you have a 2% stock...
the amount of strep in the plates is 100 ug/ml. if you have a 2% stock solution of streptomycin, how much would you need to add to prepare 1 litre of this medium?
Homework Answers
Answer-
Stock solution of streptomycin = 2%(2g in 100 ml)
= 0.02g/ ml
=20mg/ml
C1 =20,000ug/ml
C2 = 100ug/ ml
V2= 1L = 1000ml
V1= ?
we can calculate amount of stock solution of streptomycin should be added in 1L of concentration 100ug/ml-
C1×V1=C2×V2
20,000×V1= 100× 1000
V1= (100×1000)÷20,000
=1,00,000÷20,000
V1=5ml
The 5ml of streptomycin stock solution is added to 995ml of this medium to prepare plates of 1000ml media with streptomycin concentration 100ug/ml.
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