Question

What properties of RNA polymerase are enhanced by the factors that join the enzyme during the elongation phase of transcription? How are these factors recruited to the polymerase during the shift from the initiation to the elongation phase?

Answer 1

TRANSCRIPTION CONSISTS OF THREE PROCESSES -INITIATION ,ELONGATION AND TERMINATION.RNA POLYMERASE IS ONLY CAPABLE OF CATALYSING THE PROCESS OF ELONGATION.IT ASSOCIATES WITH TWO FACTORS TO INITIATE AND TERMINATE THE TRANSCRIPTION.FOR INITIATING THE TRANSCRIPTION IT ASSOCIAYES WITH THE INITIATION FACTOR FOR TERMINATING THE TRANSCRIPTION IT ASSOCIATES WITH THE TERMINATION FACTOR.ASSOCIATION OF THESE TWO FACTORS CHANGE THE SPECIFICITY OF RNA POLYMERASE TO INITIATE OR TERMINATE THE TRANSCRIPTION.THE PROPERTY OF INITIATION IS ENHANCED BY THE INITIATION FACTOR AND PROPERTY OF TERMINATION IS ENHANCED BY THE TERMINATION FACTOR.THE INITIATION FACTOR BINDS WITH THE RNA POLYMERASE AT THE PROMOETR REGION AND TERMINATION FACTOR BINDS WITH RNA POLYMERASE AT THE TERMINATOR REGION.INITIATION FACTOR WILL HELP THE RNA POLYMERASE TO RECONISE THE PROMOTER REGION AND INITIATE THE TRANSCRIPTION.WHILE TERMINATOR REGION WILL HEP THE RNA POLYMERASE TO RECOGNISE THE TERMINATOR SEQUENCE