Question

What would have been needed in the PCR reaction to obtain bands for the gene that was inserted into the vector? Give 3 examples of what CRISPR can be used to do. States)

Answer 1

Answers:

A standard, or DNA ladder, is typically included so that the size of the fragments in the PCR sample can be determined. DNA fragments of the same length form a "band" on the gel, which can be seen by eye if the gel is stained with a DNA-binding dye.

i.e., a primer, Taq polymerase, and a dye is needed to obtain bands of the gene that was interested in the vector.

Examples of CRISPR

1) CRISPR associated nucleases have shown to be useful as a tool for molecular testing due to their ability to specifically target nucleic acid sequences in a high background of non-target sequences

2) CRISPR technology has been applied in the food and farming industries to engineer probiotic cultures and to immunize industrial cultures (for yogurt, for instance) versus infections. It is also being used in crops to enhance yield, drought tolerance, and nutritional homes.

3) Cas9 enzymes together with CRISPR sequences form the basis of a technology known as CRISPR-Cas9 that can be used to edit genes within organisms. This editing process has a wide variety of applications including basic biological research, development of biotechnology products, and treatment of diseases.

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