Question

Question 1: Saved Advantages of the Polymerase Chain Reaction include all of these, except:

1) The reaction is specific for certain sequences in the DNA.

2) Only small amounts of template are needed.

3) Results can only be obtained with freshly isolated DNA.

4) All the products from a specific part of the DNA will be the same size.

Question 2: Saving The chain terminator used in Sanger's DNA sequencing is works due to..

1) lack of 2'OH

2) ddNTP: lack of 2'OH

3) dNTPlack of 3'OH

4) ddNTP: lack of 3'OH

Question 3: The following steps are all involved in genetic recombination

1.) Screening for that contains the recombined gene

2.) Cutting the vector with restriction enzymes.

3.) Mixing the gene of interest with the vector

4.) Isolating the gene of interest from its original source Ligating the gene of interest and the vector together The following sequence of these tive steps would be typical

Question 4: Coronavirus is a type of Virus The genome can be detected by

1 RNA, PCR

2) DNA, PCR

3) RNA, RT -PCR ( Reverse transcription PCR )

4) RT- PCR

Question 5: Which of the following are methods by which cells can be induced to take up recombinant DNA molecules ?

1 ) Heat shock

2) DNA "guns" which spray the DNA at very high speeds

3) Electroporation

4) Phage or virus infection.

5) All of these are used to transform bacteria.

Question 6: Multiple cloning site (MCS) in most commercially available plasmids offer all the following advantages over the earlier plasmids used in genetic recombination except:

1) They assure that only the desired DNA will be inserted into the plasmid.

2) They can be used with a wide variety of restriction enzymes

3) They enable easy control of the direction of insertion of the gene of interest.

4) They are usually adjacent to a promoter for gene expression

Question 7: Migration of linear DNA during electrophoresis is based

1) mostly on the charge-to- mass ratio since this can vary greatly among pieces of DNA

2 ) mostly on the size of the molecule, since the ratio of charge to mass is approximately the same, no matter how large the DNA

3) mostly on the charge of the molecule, since the ratio of charge to mass is approximately the same, no matter how large the DNA ,

4) on parameters not understood well.

Answer 1

Answer:

1. (3) Result can be obtained with old DNA but it should be free of contamination.

2.(4) ddNTP lacking 3' OH - which results in chain termination due to lack of bond formation.

3. Sequence

-cutting the vectors with restriction enzymes

-Isolating the gene of interest from its original source and ligating the gene of interest and vector together.

-mixing the gene of interest with the vector.

-screening for that contains the combined gene.

4.(3)Corona is a RNA virus and hence can be detected by Reverse transcriptase PCR.

5. (5)All of these are used to transform bacteria.

6.(3)They don't enable the the easy insertion of orientation.

7(2) Generally DNA is negatively charged. So it depends mainly on the size.

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