Question

B. Reaction mechanisms (media, reagents, enzymes, products) 1. Media. Match the name of medium with the physiological test. For some tests, multiple media can be used. a. Kligler's iron agar b. MR-VP broth c. Bromocresol Purple (BCP) lactose d. SIM medium e. Simmon's citrate agar f. Skim milk agar 8. Spirit Blue agar h. Tryptone broth _2,3 butanediol fermentation carbohydrate fermentation _casein hydrolysis citrate utilization hydrogen sulfide production mixed acid fermentation triglyceride hydrolysis _tryptophan degradation

Answer 1

a. Klingler's iron agar-- 1. hydrogen sulphide production

2. carbohydrate fermentation

b. Mr-VP broth - - 1.mixed acid fermentation

2. 2,3 butanediol fermentation

c. Bromocresol purple lactose-- 1.carbohydrate fermentation

d. SIM medium-- 1.Hydrogen sulphide production

e. Simmon's citrate agar-- 1.citrate utilisation.

f. Skim milk agar-- 1.casein hydrolysis

g. Spirit blue agar-- 1.triglyceride hydrolysis

h. Tryptone broth-- 1.tryptophan degradation.