Question

I will be negalite control a positive control * Homework before The Hill Reaction Lab, your group should do the following: 1) Write a question that you would like to answer about how different conditions alleet the Hill reaction. Remember, a possible answer to this question should be your hypothesis, not predictions about the specific outcome of an experiment Here is an example: Question: Is there a specific temperature range within which photosynthesis can continue to operate? Hypothesis: There is a narrow range of temperatures within which photosynthesis will work. Prediction: If the temperature of the reaction is raised to 85 degrees, the rate of the Hill reaction will decrease compared with the room temperature positive control 2) Write a hypothesis in response to the question that you are asking. Remember that hypotheses are tentative answers to the questions that you posed. While it might seem obvious to you which hypothesis will be supported by your experimental results, as good scientists we should be open to the possibility of unexpected results. One way to do this is to think in advance about alternative outcomes. 3) Write out one prediction for your hypothesis. This prediction should direct you to an experimental design that will let you test your prediction. Predictions are usually in the form of, "if I change X in a particular way the result will be Y." 4) Sketch out an experimental design that will allow you to test your predictions. Make sure you include appropriate controls in your experiment. ERO 5) Discuss with your instructor the justification for your hypothesis, predictions, and choice of controls. Isolated chloroplast add mite light, waslelength of light, we have to explain why Im lemperature, high temperature or cold tomperet w concentration of amonía Will eliminate

Answer 1

1. Hypothesis or question: Propose a testable hypothesis concerning the Hill reaction.

-If cobalt and chlorine has less chloroplast than spinach, then less photosynthesis will occur.

HYPOTHESIS

From: What Kind of Food contains a High Amount of Chlorophyll

A deficiency of cobalt and chlorine, in some way induces changes which result in chloroplasts with reduced Hill reaction activity per unit of chlorophyll. PREDICTION

2. Chloroplasts can be reactivated by the deficient nutrient cobalt and chlorine in vitro, it can be concluded that the lowered activities observation were not due to lack of the deficient nutrient ion in the Hill reaction.

3. If we change small amount of Ph of cobalt and chlorine there will be increased Hill reaction activity per unit of chlorophyll.

4. Among the nutrients, deficiencies of cobalt and chlorine caused impairment of activity in that decreasing order of severity.

In no case was the activity of chloroplasts from a deficient plant enhanced by the addition of the missing nutrient to the chloroplast suspension.

A comparison of the different ph with different diluting factor of chloroplasts from healthy and deficient plants.

In some deficiencies a small but significant decrease was observed in the ratio of chlorophyll a to chlorophyll b.

Chloroplasts from healthy and deficient plants were assayed over a range of limiting light intensities up to saturation, and the effect of ph on the different dilution was recorded.

In some cases, the different dilution due to deficiency was measured at 30 and 10°C with saturating light intensity.