target dna is integrated into a plasmid inserted into bacteria and allows for selection of transformed...
target dna is integrated into a plasmid inserted into bacteria and allows for selection of transformed bacteria colonies
Homework Answers
Transformation is a key step in DNA cloning. DNA of interest is cut down by restriction enzymes and then ligated into a plasmid and then this plasmid is tranferred into the bacteria. Bacteria are selected on antibiotic plates. Bacteria with a plasmid are antibiotic-resistant, and each one will form a colony.
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target dna is integrated into a plasmid inserted into
bacteria and allows for selection of transformed...
Antibiotic selection allows you to determine which bacteria contain the plasmid of interest, but do not...
Antibiotic selection allows you to determine which bacteria contain the plasmid of interest, but do not necessarily tell you if the plasmid has taken up the recombinant DNA and contains your gene of interest. Which gene, which spans the multiple cloning site (MCS), allows you to screen for plasmids that contain your insert? A. β-lactamase (bla) B. Ori C. lacZ D. X-gal
We added IPTG and X-gal to the LB plates for the selection of the plasmid transformed...
We added IPTG and X-gal to the LB plates for the selection of the plasmid transformed bacteria. What is the function of IPTG and X-gal?
1. Why do plasmid vectors contain a polylinker? So that the plasmid can be cut into...
1. Why do plasmid vectors contain a polylinker? So that the plasmid can be cut into many small fragments to be inserted in the region of interest To allow the plasmid to survive on antibiotic media To find a restriction enzyme site that is useful for inserting the fragment of interest Multiple cut sites are required for the DNA fragment to be inserted. 2.What does blue color mean in blue-white screening with pUC18? The DNA fragment was successfully inserted into...
A plasmid containing recombinant DNA is introduced into a bacteria. This plasmid is cloned during... a....
A plasmid containing recombinant DNA is introduced into a bacteria. This plasmid is cloned during... a. metaphase I b. binary fission c. the S phase of interphase d. mitosis
P. lac MYC coding sequence (fig73) amp ori You have successfully cloned MYC into the plasmid,...
P. lac MYC coding sequence (fig73) amp ori You have successfully cloned MYC into the plasmid, as shown in the figure above. Using your understanding of plasmids and protein expression in bacteria, complete the table below: You have successfully cloned MYC into the plasmid, as shown in the figure above. Using your understanding of plasmids and protein expression in bacteria, complete the table below: condition colonies or no colonies? protein expression or no protein expression? < transformed with desired plasmid;...
The purpose of including an ampicillin resistance gene in a plasmid into which you are cloning...
The purpose of including an ampicillin resistance gene in a plasmid into which you are cloning a piece of DNA is: This is used to prevent expression of other ampicillin resistance genes in the bacterial genome. This enables the creation of ampicillin-resistant bacteria for biomedical research. This enables selecting a bacteria colony transformed with a plasmid with a DNA insert ligated in as a means of amplifying a specified piece of DNA. This enables selecting a bacterial colony transformed with...
help asap please The purpose of including an ampicillin resistance gene in a plasmid into which...
help asap please
The purpose of including an ampicillin resistance gene in a plasmid into which you are cloning a piece of DNA is: This is used to prevent expression of other ampicillin resistance genes in the bacterial genome. This enables the creation of ampicillin-resistant bacteria for biomedical research. ChmThis enables selecting a bacteria colony transformed with a plasmid with a DNA insert ligated in as a means of amplifying a specified piece of DNA. This enables selecting a bacterial...
You isolate plasmid DNA from bacteria (Questions 7-10) 7) A plasmid is an extrachromosomal circul...
10 please and 7
You isolate plasmid DNA from bacteria (Questions 7-10) 7) A plasmid is an extrachromosomal circular DNA frequently found in prokaryotes. Aside from being smaller, how is it different from the prokaryotic genome? You place equal amounts of plasmid DNA in 4 different tubes and incubate the DNA with increasing amounts of the enzyme topoisomerase I for 1 hour (0 enzyme units 0.25 enzyme units, 0.5 enzyme units and 1 enzyme unit). You then analyze the plasmid...
Multiple Choice, Please answer all 4 What is the purpose of the neutralization solution when purifying...
Multiple Choice, Please answer all 4 What is the purpose of the neutralization solution when purifying plasmid DNA? precipitates plasmid DNA lyses the cells contains a detergent precipitates chromosomal DNA and proteins Why does the DNA bind to the purification resin? the resin is negatively charged the resin is hydrophobic the resin is positively charged the resin is neutral Why did you get colonies on plate A but not on B in the transformation, even though both plates had ampicillin?...
"In genetic modifications, DNA inserted can come from _____ ." any organism containing the DNA of...
"In genetic modifications, DNA inserted can come from _____ ." any organism containing the DNA of interest only the same plasmid as the vector only the same species as the vector only the same species as the host only the same phylum as the plasmid vector
P. lac MYC coding sequence (fig73) amp ori You have successfully cloned MYC into the plasmid, as shown in the figure above. Using your understanding of plasmids and protein expression in bacteria, complete the table below: You have successfully cloned MYC into the plasmid, as shown in the figure above. Using your understanding of plasmids and protein expression in bacteria, complete the table below: condition colonies or no colonies? protein expression or no protein expression? < transformed with desired plasmid;...
The purpose of including an ampicillin resistance gene in a plasmid into which you are cloning a piece of DNA is: This is used to prevent expression of other ampicillin resistance genes in the bacterial genome. This enables the creation of ampicillin-resistant bacteria for biomedical research. This enables selecting a bacteria colony transformed with a plasmid with a DNA insert ligated in as a means of amplifying a specified piece of DNA. This enables selecting a bacterial colony transformed with...
help asap please
The purpose of including an ampicillin resistance gene in a plasmid into which you are cloning a piece of DNA is: This is used to prevent expression of other ampicillin resistance genes in the bacterial genome. This enables the creation of ampicillin-resistant bacteria for biomedical research. ChmThis enables selecting a bacteria colony transformed with a plasmid with a DNA insert ligated in as a means of amplifying a specified piece of DNA. This enables selecting a bacterial...
10 please and 7
You isolate plasmid DNA from bacteria (Questions 7-10) 7) A plasmid is an extrachromosomal circular DNA frequently found in prokaryotes. Aside from being smaller, how is it different from the prokaryotic genome? You place equal amounts of plasmid DNA in 4 different tubes and incubate the DNA with increasing amounts of the enzyme topoisomerase I for 1 hour (0 enzyme units 0.25 enzyme units, 0.5 enzyme units and 1 enzyme unit). You then analyze the plasmid...
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