We added IPTG and X-gal to the LB plates for the selection of the plasmid transformed...
We added IPTG and X-gal to the LB plates for the selection of the plasmid transformed bacteria. What is the function of IPTG and X-gal?
Homework Answers
IPTG is used along with X-gal for the purpose of blue-white screening. It is a non-metabolizable, which induces the expression of lacZ gene. Chromogenic substrate such as X-gal is required for visual screening purposes and used in visual indication.
Add Answer to:
We added IPTG and X-gal to the LB plates for the selection of
the plasmid transformed...
target dna is integrated into a plasmid inserted into bacteria and allows for selection of transformed...
target dna is integrated into a plasmid inserted into bacteria and allows for selection of transformed bacteria colonies
P. lac MYC coding sequence (fig73) amp ori You have successfully cloned MYC into the plasmid,...
P. lac MYC coding sequence (fig73) amp ori You have successfully cloned MYC into the plasmid, as shown in the figure above. Using your understanding of plasmids and protein expression in bacteria, complete the table below: You have successfully cloned MYC into the plasmid, as shown in the figure above. Using your understanding of plasmids and protein expression in bacteria, complete the table below: condition colonies or no colonies? protein expression or no protein expression? < transformed with desired plasmid;...
Question 10 0.5 pts Thinking carefully about the genes on the plasmid, and the effect of...
Question 10 0.5 pts Thinking carefully about the genes on the plasmid, and the effect of arabinose on the expression of GFP, predict what will happen when we plate bacteria that are TRANSFORMED on plates containing ampicillin, but NO arabinose. the bacteria will grow and glow the bacteria will grow, but won't glow the bacteria will not grow Question 11 0.5 pts Thinking carefully about the genes on the plasmid, and the effect of arabinose on the expression of GFP,...
This is what we did in the experiment .First of all,1 µl of plasmid DNA was...
This is what we did in the experiment .First of all,1 µl of
plasmid DNA was added into the tube which contains competent cells
and the tube was tapped gently to mix DNA and the competent
bacteria. After that it was placed on ice for 30 minutes. Then, the
tube with the competent bacteria and plasmid were transferred to
heating block at 42 °C and the tube was leaved in there exactly 90
seconds. 0.25 ml of LB broth was...
Justify please! DISCUSSION POST #2 If bacteria are grown on plates with ampicillin and X-gal, explain...
Justify please!
DISCUSSION POST #2 If bacteria are grown on plates with ampicillin and X-gal, explain which color colonies, white or blue, would contain bacteria with recombinant plasmid.
This is what we did in the experiment .First of all,1 µl of plasmid DNA was...
This is what we did in the experiment .First of all,1 µl of
plasmid DNA was added into the tube which contains competent cells
and the tube was tapped gently to mix DNA and the competent
bacteria. After that it was placed on ice for 30 minutes. Then, the
tube with the competent bacteria and plasmid were transferred to
heating block at 42 °C and the tube was leaved in there exactly 90
seconds. 0.25 ml of LB broth was...
E. coli strain BL21(DE3) was transformed with the pET30a+ plasmid containing the ilvC gene that encodes for E. coli KARI. The starter culture was incubated overnight in LB broth containing 50 µg/mL ka...
E. coli strain BL21(DE3) was transformed with the pET30a+ plasmid containing the ilvC gene that encodes for E. coli KARI. The starter culture was incubated overnight in LB broth containing 50 µg/mL kanamycin at 37°C. The sample were already added IPTG and the OD600 reaches the 0.6-0.7 range. How to improve the purity and yield ?
Principle of blue- white screening method(why do the colonies appear blue or white? what do we...
Principle of blue- white screening method(why do the colonies appear blue or white? what do we try to recover?what are the roles of substrate (Xgal) and the gene expression inducer(IPTG)?) this experiment was GENETIC DEFECT CORRECTION WITH BACTERIAL TRANSFORMATION In this experiment we did that first of all,1 µl of plasmid DNA was added into the tube which contains competent cells and the tube was tapped gently to mix DNA and the competent bacteria.After that it was placed on ice...
Antibiotic selection allows you to determine which bacteria contain the plasmid of interest, but do not...
Antibiotic selection allows you to determine which bacteria contain the plasmid of interest, but do not necessarily tell you if the plasmid has taken up the recombinant DNA and contains your gene of interest. Which gene, which spans the multiple cloning site (MCS), allows you to screen for plasmids that contain your insert? A. β-lactamase (bla) B. Ori C. lacZ D. X-gal
1. Fill in the table above with what you observe on your plates. 2. Bacterial transformation...
1. Fill in the table above with what you observe on your
plates.
2. Bacterial transformation occurred on which agar plate (s)?
What evidence do you have that the bacteria were transformed
here?
3. Which plates have glowing growth? Explain what causes
bacteria to glow.
II. Transformation of E. coli with Plasmid DNA (PGLO) 1. Three LB (Luria Broth) agar plates are obtained. The plates contain: • Plate A: LB-Agar/Ampicillin/Arabinose • Plate B: LB-Agar/Ampicillin • Plate C: LB-Agar 2. Three...
P. lac MYC coding sequence (fig73) amp ori You have successfully cloned MYC into the plasmid, as shown in the figure above. Using your understanding of plasmids and protein expression in bacteria, complete the table below: You have successfully cloned MYC into the plasmid, as shown in the figure above. Using your understanding of plasmids and protein expression in bacteria, complete the table below: condition colonies or no colonies? protein expression or no protein expression? < transformed with desired plasmid;...
Question 10 0.5 pts Thinking carefully about the genes on the plasmid, and the effect of arabinose on the expression of GFP, predict what will happen when we plate bacteria that are TRANSFORMED on plates containing ampicillin, but NO arabinose. the bacteria will grow and glow the bacteria will grow, but won't glow the bacteria will not grow Question 11 0.5 pts Thinking carefully about the genes on the plasmid, and the effect of arabinose on the expression of GFP,...
This is what we did in the experiment .First of all,1 µl of
plasmid DNA was added into the tube which contains competent cells
and the tube was tapped gently to mix DNA and the competent
bacteria. After that it was placed on ice for 30 minutes. Then, the
tube with the competent bacteria and plasmid were transferred to
heating block at 42 °C and the tube was leaved in there exactly 90
seconds. 0.25 ml of LB broth was...
Justify please!
DISCUSSION POST #2 If bacteria are grown on plates with ampicillin and X-gal, explain which color colonies, white or blue, would contain bacteria with recombinant plasmid.
This is what we did in the experiment .First of all,1 µl of
plasmid DNA was added into the tube which contains competent cells
and the tube was tapped gently to mix DNA and the competent
bacteria. After that it was placed on ice for 30 minutes. Then, the
tube with the competent bacteria and plasmid were transferred to
heating block at 42 °C and the tube was leaved in there exactly 90
seconds. 0.25 ml of LB broth was...
1. Fill in the table above with what you observe on your
plates.
2. Bacterial transformation occurred on which agar plate (s)?
What evidence do you have that the bacteria were transformed
here?
3. Which plates have glowing growth? Explain what causes
bacteria to glow.
II. Transformation of E. coli with Plasmid DNA (PGLO) 1. Three LB (Luria Broth) agar plates are obtained. The plates contain: • Plate A: LB-Agar/Ampicillin/Arabinose • Plate B: LB-Agar/Ampicillin • Plate C: LB-Agar 2. Three...
Most questions answered within 3 hours.
-
A χ2-curve, looking at the relationship between age and hours
spent working at an office per...
asked 1 minute ago -
The pH of a sample of water from a river is 5.0. A
sample of effluent from...
asked 46 minutes ago -
At the beginning of the period, the Fabricating Department
budgeted direct labor of $136,500 and equipment...
asked 1 hour ago -
Please answer all
____ 28. Rent control is usually
justified on the grounds that it protects...
asked 1 hour ago -
PARTS A-D HAVE BEEN ANSWERED. WAS TOLD TO REPOST. ONLY ANSWER
PARTS E and F.
A...
asked 1 hour ago -
2) You are given the task of finding a representation for a
circle in a drawing...
asked 2 hours ago -
STUDY QUESTION: Does use of diet drug fen-phen
(fenfluramine-phentermine) cause valvular heart disease?
HINT: Valvular heart...
asked 2 hours ago -
1. An object weighing 40 N rests on a surface. The coefficient
of friction is 0.35....
asked 3 hours ago -
Investor company owns 35% of investee company voting stock and
accounts for the investment under the...
asked 4 hours ago -
The number of major faults on a randomly chosen 1 km stretch of
highway has a...
asked 5 hours ago -
Consider the competitive environment of Starbuck's, Progressive
Insurance, a manufacturing firm with low turnover, or a...
asked 6 hours ago -
3. Gains from trade
Consider two neighbouring island countries called Euphoria and
Contente. They each have...
asked 7 hours ago