Question

2. What would happen to each of the following if reduced the supply of glucose to cells by 50%? You may assume that the cell will NOT switch to an alternative energy source. Briefly explain why for each. A. The level of glycolysis activity. B. The level of Krebs Cycle activity. C. The level of activity in the electron transport chain in the mitochondria. D. The size of the proton gradient across the inner mitochondrial membrane. E. The rate of ATP synthesis by chemiosmosis F. The rate of O2 use (reduction to water in electron transport)

Answer 1

The glycolysis activity will be reduced rather gluconeogenesis wi be happened.

Krebs cycle can be increased because it can be continued without break down of glucose it can be by beta oxidation of fat which will produce many acetyl co A molecules and this step will give energy supply.

It will be increased because fatty acid oxidation give more acetyl co A and thus it will produce more NADH AND FADH2 which will be oxidized and makes ATP.

PROTON GRADIENT IS SAME BECAUSE IT REMAIN SAME AND REDUCES WHEN ATP IS FORMED AND AGAIN IT BUILDS UP WHEN NEW ELECTRON BEING TRANSPORTED.

E) WILL BE INCREASED.

3.A) REDUCED ATP WILL BR PRODUCED BECAUSE THIS GRADIENT DRIVE THE MOVEMENT OF H+ IONS INTO MITOCHONDRIAL MATRIX AND THROUGH ATP SYNTHASE AND MAKES ATP.

B)RATE OF ATP SYNTHESIS WILL ALSO BE REDUCED.

C) OXIDATION OF NADH WILL NOT BE HAPPENED .

D)THE LEVEL OF NAD+ WILL BE REDUCED AS MORE REDUCED NAD WHICH IS NADH WILL BE PRESENT.

E)THE RATE OF KREB'S CYCLE ACTIVITY WILL BE REDUCED BECAUSE MANY RATE LIMINTUNG STEP OF KREB'S LIKE PFH COMPLEX AND CITATRE SYNTHASE IS NEGATIVELY CONTROLLED BY NADH.

F) GLYCOLYSIS ACTIVITY WILL ALSO BE INCREASED BECAUSE YHE CE WILL HAVE A TREMENDOUS REQUIREMENTS OF ATP.

G) FERMENTATION WILL BE MORE.

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