Question (8) Concerning the passage of cells in tissue culture, trypsin is used to: Remove the...
Question (8) Concerning the passage of cells in tissue culture, trypsin is used to:
- Remove the residual proteins in the serum
- Detach the cells from the tissue culture plastic
- As an energy source for the cells
- Remove toxins from the residual spent medium
- Encourage cell proliferation
Homework Answers
Trypsin is a serine protease enzyme helps to detach the adherent cells from tissue culture flask. Trypsinization is the process of cell dissociation using trypsin, a proteolytic enzyme which breaks down proteins, to dissociate adherent cells from the vessel in which they are being cultured. When added to a cell culture, trypsin breaks down the proteins which enable the cells to adhere to the vessel. Trypsinization is often used to pass cells to a new vessel. When trypsinization process is complete the cells will be in suspension and appear rounded.
Therefore Option B is correct.
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Question (8) Concerning the passage of cells in tissue culture,
trypsin is used to:
Remove the...
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thats all the information that he gave us to solve the question. Thank you for trying...
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Thank you for trying anyways
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B.4. John used cDNA microarrays from a company to study the oxygen effects on metabolic gene expression patterns by culturing hepatocytes in different incubators with 5%, 20% or 40% oxygen concentrations. Cells in 20% oxygen incubator were used as control samples. Then he isolated DNA samples for these three culture conditions and followed the protocol from the manufacturer beginning from sample and chip hybridization to data analysis. However,...
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I need help ASAP pleaseeee it is due in 30mins
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explain figure 2 please on the third page
The Jounal of Immunol nes of the National Academy of Sciences USA 89. 6550-6554. 01992, by permision of Proc. Natl. Acad. Sci. USA Vol. 89, pp. 6550-63554, July 1992 Immunolog A 39-kDa protein on activated helper T cells binds CD40 and transduces the signal for cognate activation of B cells RANDOLPH J. NOELLE时, MEENAKSHI ROY.. DAVID M. SHEPHERD., IVAN STAMENKOVICt JEFFREY A. LEDBETTER, AND ALEJANDRO ARUFFo Departmend Microbiology. Dartmouth Medical School One...
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thats all the information that he gave us to solve the question.
Thank you for trying anyways
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