For the following gene, MspI and HpaII
restriction sites and their positions are indicated here.
HpaII is methylation sensitive, while MspI is
not.
If this gene is being actively transcribed, how many fragments would you expect from this double restriction digest?
6
3
5
4
1
Homework Answers
Answer
6 fragments expected
For the following gene, MspI and HpaII restriction sites and
their positions are indicated here. HpaII is methylation sensitive,
while MspI is not.
If this gene is being actively transcribed, how many fragments would you expect from this double restriction digest
6 fragments possible
Add Answer to:
For the following gene, MspI and HpaII
restriction sites and their positions are indicated here.
HpaII...
please help me with these questions Lab 8 Extension Activity: Plasmid Mapping and Restriction Enzymes Mapping...
please help me with these questions
Lab 8 Extension Activity: Plasmid Mapping and Restriction Enzymes Mapping the Plasmid The first step in mapping a plasmid is to determine how many times a restriction site is found on that plasmid. Examine the results for plasmid 55 as an example. The data given in the following table are for the double digest using EcoRI and Pstl. Also, giving are the data for single digests by the individual enzymes. The numbers in the...
In your previous prac session you digested your POTC-A plasmid DNA with 3 enzyme mixes (AB...
In your previous prac session you digested your POTC-A plasmid DNA with 3 enzyme mixes (AB and C). One mix contained the restriction endonuclease Kpnl alone, another contained both del and Not and another contained Sphi and Nhe, but you don't know which was which yet. The sites where these enzymes cut POTC-A are indicated on the plasmid map at the top of the page. Calculate the sizes of the DNA fragments that you would expect to see on your...
A plasmid is cleaved by restriction endonucleases and analyzed by agarose gel electrophoresis. Assume there is...
A plasmid is cleaved by restriction endonucleases and analyzed
by agarose gel electrophoresis. Assume there is no supercoiling.
Answer the following three questions about the plasmid.
Can you please help below? I also don't understand it so a
explanation for each would be helpful. For the first one I think
its 1000bp but unsure. For the second, I think there is 2 HindIII
sites and for the last one there is 1 EcoRI site? Please
explain.
A plasmid is cleaved...
question number 7 purifying plasmid from your cells, you will set up a teaction to cut...
question number 7
purifying plasmid from your cells, you will set up a teaction to cut the plasmid DNA with the restriction enzyme Bgl Il. See the map below for the positions of the Bgl Il restriction enzyme sites on the plasmid. How many DNA fragments would you expect if your restriction enzyme digest was complete and your plasmid contains insert? Question 7 Not yet graded/ 1 pts After you electrophoresed the plasmid digest in Question 6, your gel showed...
Use the diagram below for questions 9-10. The black line indicates a locus, arrows indicate restriction sites and the red line indicates a probe to the region indicated. What does the arrow with the...
Use the diagram below for questions 9-10. The black line indicates a locus, arrows indicate restriction sites and the red line indicates a probe to the region indicated. What does the arrow with the star/asterisk above it mean? Choose all possible answers. All of these answers are correct. None of these answers are correct. That restriction site is a SNP. That restriction site is a RFLP. That site will not vary between homologs within an individual. That site will not...
Restriction Mapping Below is a restriction map for the plasmid PGEN101 (total length - 20 kb)....
Restriction Mapping Below is a restriction map for the plasmid PGEN101 (total length - 20 kb). Using this map as a guide, give the number of restriction fragments along with their associated lengths that would result from digesting PGEN101 with the restriction enzymes EcoRI, BamHII, anda combination of EcoRI + BamHI. BamHI BamHI BamHI / PGEN101 (20 kb) Mb EcoRI Digest Performed Size Emments Obtained EcoRI........ BamHI.. EcoRI + BamHI.... Two freshmen college students, interested in becoming gene jocks, performed...
on the me but not with the larger fragments on o t and more and record...
on the me but not with the larger fragments on o t and more and record med in the h 3. Determine the distracted for each bund data bere 4. Determine the distance traveled for each and generated in the double digest and record data bere tion Endonuclease Digestion and Gel Electrophoresis of DNA 185 VI. RESTRICTION MAPPING The different DNA fragments generated by different map DNA. For example, specific DNA on 2000 nucleotides in las Rl or Hind and...
Below is a schematic of gene Hemoglobin beta subunit (HBS), which encodes protein HBS. The promoter...
Below is a schematic of gene Hemoglobin beta subunit (HBS), which
encodes protein HBS. The promoter region is indicated by the dotted
box. Transcriptions begins immediately following the promoter. The
mature mRNA produced by this gene would be approximately how many
nucleotides long?
A) 100
B) 200
C) 3000
D) 5000
E) 7000
1. Below is a schematic of gene Hemoglobin beta subunit (HBS), which encodes protein HBS. The promoter region is indicated by the dotted box. Transcription begins immediately...
The extracted plasmid DNA is pUC19. Look up the plasmid map and include in your pre-lab...
The extracted plasmid DNA is pUC19. Look up the plasmid map and
include in your pre-lab as well as your report.
a. What is the location of the BamH1 restriction site? Xmn1
restriction site? (sequence location by number). How many of these
sites exist each?
b. If single digestions were performed (one restriction enzyme)
with BamH1 and Xmn1, respectively, how many fragments would form,
and what would be the sizes of each of these fragments?
c. If a double digestion...
A biology 207 student sub cloned the promoterless, GFP gene from pCamR-GFP into pUC18-207 using a...
A biology 207 student sub cloned the promoterless, GFP gene from pCamR-GFP into pUC18-207 using a double digest. They used competent, prototrophic DH5aE. coli cells in their transformation protocol and plated the transformation reaction onto minimal medium that contained additional supplements to help them screen and select for their desired transformants, which are transformed E. coli cells that express the GFP gene. All experimental procedures were followed such as incubation times etc. Note that GFP encodes for a protein that...
please help me with these questions
Lab 8 Extension Activity: Plasmid Mapping and Restriction Enzymes Mapping the Plasmid The first step in mapping a plasmid is to determine how many times a restriction site is found on that plasmid. Examine the results for plasmid 55 as an example. The data given in the following table are for the double digest using EcoRI and Pstl. Also, giving are the data for single digests by the individual enzymes. The numbers in the...
In your previous prac session you digested your POTC-A plasmid DNA with 3 enzyme mixes (AB and C). One mix contained the restriction endonuclease Kpnl alone, another contained both del and Not and another contained Sphi and Nhe, but you don't know which was which yet. The sites where these enzymes cut POTC-A are indicated on the plasmid map at the top of the page. Calculate the sizes of the DNA fragments that you would expect to see on your...
A plasmid is cleaved by restriction endonucleases and analyzed
by agarose gel electrophoresis. Assume there is no supercoiling.
Answer the following three questions about the plasmid.
Can you please help below? I also don't understand it so a
explanation for each would be helpful. For the first one I think
its 1000bp but unsure. For the second, I think there is 2 HindIII
sites and for the last one there is 1 EcoRI site? Please
explain.
A plasmid is cleaved...
question number 7
purifying plasmid from your cells, you will set up a teaction to cut the plasmid DNA with the restriction enzyme Bgl Il. See the map below for the positions of the Bgl Il restriction enzyme sites on the plasmid. How many DNA fragments would you expect if your restriction enzyme digest was complete and your plasmid contains insert? Question 7 Not yet graded/ 1 pts After you electrophoresed the plasmid digest in Question 6, your gel showed...
Use the diagram below for questions 9-10. The black line indicates a locus, arrows indicate restriction sites and the red line indicates a probe to the region indicated. What does the arrow with the star/asterisk above it mean? Choose all possible answers. All of these answers are correct. None of these answers are correct. That restriction site is a SNP. That restriction site is a RFLP. That site will not vary between homologs within an individual. That site will not...
Restriction Mapping Below is a restriction map for the plasmid PGEN101 (total length - 20 kb). Using this map as a guide, give the number of restriction fragments along with their associated lengths that would result from digesting PGEN101 with the restriction enzymes EcoRI, BamHII, anda combination of EcoRI + BamHI. BamHI BamHI BamHI / PGEN101 (20 kb) Mb EcoRI Digest Performed Size Emments Obtained EcoRI........ BamHI.. EcoRI + BamHI.... Two freshmen college students, interested in becoming gene jocks, performed...
on the me but not with the larger fragments on o t and more and record med in the h 3. Determine the distracted for each bund data bere 4. Determine the distance traveled for each and generated in the double digest and record data bere tion Endonuclease Digestion and Gel Electrophoresis of DNA 185 VI. RESTRICTION MAPPING The different DNA fragments generated by different map DNA. For example, specific DNA on 2000 nucleotides in las Rl or Hind and...
Below is a schematic of gene Hemoglobin beta subunit (HBS), which
encodes protein HBS. The promoter region is indicated by the dotted
box. Transcriptions begins immediately following the promoter. The
mature mRNA produced by this gene would be approximately how many
nucleotides long?
A) 100
B) 200
C) 3000
D) 5000
E) 7000
1. Below is a schematic of gene Hemoglobin beta subunit (HBS), which encodes protein HBS. The promoter region is indicated by the dotted box. Transcription begins immediately...
The extracted plasmid DNA is pUC19. Look up the plasmid map and
include in your pre-lab as well as your report.
a. What is the location of the BamH1 restriction site? Xmn1
restriction site? (sequence location by number). How many of these
sites exist each?
b. If single digestions were performed (one restriction enzyme)
with BamH1 and Xmn1, respectively, how many fragments would form,
and what would be the sizes of each of these fragments?
c. If a double digestion...
A biology 207 student sub cloned the promoterless, GFP gene from pCamR-GFP into pUC18-207 using a double digest. They used competent, prototrophic DH5aE. coli cells in their transformation protocol and plated the transformation reaction onto minimal medium that contained additional supplements to help them screen and select for their desired transformants, which are transformed E. coli cells that express the GFP gene. All experimental procedures were followed such as incubation times etc. Note that GFP encodes for a protein that...
Most questions answered within 3 hours.
-
Assume Kw = 1.01 ✕ 10−14
For pure water, we can calculate [H3O+ ] = [OH...
asked 53 minutes ago -
Suppose that on a temperature scale X, water boils at 203.0°X
and freezes at -105.7°X. What...
asked 1 hour ago -
BaS crystallizes in a cubic unit cell with S2- ions on each
corner and each face....
asked 2 hours ago -
A. 0≤P(Oi)≤10≤P(Oi)≤1 for each i
B. P(Oi)≤0P(Oi)≤0
C. P(Oi)=1+P(OCi)P(Oi)=1+P(OiC)
D. P(Oi)≥1P(Oi)≥1
If an experiment consists of...
asked 3 hours ago -
A battery has an emf of 9.20V and an internal resistance of 1.20
ohm. a)What resistance...
asked 3 hours ago -
The area of an elastic circular loop decreases at a constant
rate, dA/dt = −6.60×10−3 m2/s...
asked 4 hours ago -
The denaturation of proteins can be described by the
equilibrium
F⇌U
where F and U represent...
asked 6 hours ago -
Please answer what the maximum and minimum force is, and the
angle on the ion is...
asked 6 hours ago -
implement a program that reads a number of rows and a symbol.
The program will draw...
asked 6 hours ago -
Assume that when adults with smartphones are randomly selected,
45% use them in meetings or classes....
asked 6 hours ago -
Determine the number of formula units of
Na2SO4 and moles of oxygen contained in 8.11
moles...
asked 6 hours ago -
Explain in steps on the following code
What would be the output when executed
using System;...
asked 6 hours ago