Bacterial morphology
E coli is a gram negative rod shaped bacterium commonly found in gut of human beings. It is having a membrane less region called nucleoid which contains the DNA. Membrane bound organelles like mitochondria, chloroplast, golgi, ER are all absent. It is a heterotrophic bacteria which obtain nutrition mainly from a living host, but mainly lives in symbiotic relationship with the host. It has 70S type ribosomes. It is peritrichous, flagella is present all over the cell surface.
Oxygen and temperature requirements
The bacterium lives at normal temperature and pH conditions. The temperature is around 37℃ which is the temperature of a healthy human body. It is an aerobic bacteria which undertakes celluar respiration in the presence of oxygen. It is a mesophytic bacterium.
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Describe bacterial morphology, oxygen and temperature requirements of Escherichia coli
Escherichia coli is a bacteria that requires oxygen for growth. Very briefly describe the conditions needed to grow 5ml of liquid culture of E.coli in the laboratory? Include 1. Temperature 2. Media 3. Oxygen requirements 4. Glassware 5. Equipment
-03] What is the advantage of growing bacteria on solid media?- 4. [CH-09-04] Describe bacterial morphology, oxygen and temperature requirements of Bacillus subtilis- 5. [CH-09-05] Describe bacterial morphology, oxygen and temperature requirements of Staphylococcus epidermidis 6. [CH-09-06] Colony size, color, and shape are critical of bacterial growth description. At least three other crucial factors - not description of the organism itself- typically included when describing bacterial growth. What are they and why are they important? 7. [CH-09-07] What steps should...
43. State four facts about DNA replication in Escherichia coli (8 points) 44. Describe the process of base excision repair in Escherichia coli (8 points)
61. Describe the morphologycell grouping, oxygen and temperature requirements for Mycobacterium smegmatis
Describe the structural features and mechanism of assembly of Escherichia coli P (Pap) pili. Use a diagram to illustrate you answer. When are these pili used during infection, and how is receptor specificity determined in this system? [ESSAY TYPE QUESTION]
There has been a wastewater spill on a beach. The Escherichia coli density of the wastewater is 7.8 x 1010 cells/100 mL. The spilled wastewater was diluted by 1000 times when mixed with seawater. The specific growth rate constant for Escherichia coli is 0.231 h-1 . Determine the density (cells/100 mL) of Escherichia coli in receiving seawater 24 and 72 hours after the spill. Note that exponential growth (X = X0e μt ) applies.
2. You are trying to design a protein that will be expressed in Escherichia coli and secreted outside of the cell for purification and use as a pharmaceutical. E. coli is a gram-negative cell and the protein folds after it has exited the cell. Which secretion system would work best for this project? Support your answer with evidence based on the properties of E. coli, the protein, and the secretion system. (20 pts) 2. You are trying to design a...
A culture of 10 bacteria of Escherichia coli has a generation time of 30 minutes at 35 degrees Celsius. After 5 hours at this temperature, how many bacteria would you expect to see?" a. "10,240" b. 50 c. "1,000" d. 320 e. "10,000,000,000"
The pathogenic strain of Escherichia coli, E. coli O 104: H4, expresses the Shiga toxin and is highly resistant to many antibiotics. How did the E. coliO104:H4 strain originally obtain the genes for the Shiga toxin and antibiotic resistance? Choose one: A. horizontal gene transfer B. vertical transmission C. random mutation D. immunopathogenesis The pathogenic strain of Escherichia coli, E. coli O 104: H4, expresses the Shiga toxin and is highly resistant to many antibiotics. How did the E. coliO104:H4...
4. Strains of Escherichia coli that cause urinary tract infections, known as Uropathogenic E. coli or UPEC, initiate infection by binding to uroepithelial cells. The E. coli fimbriae FimH binds specifically to D-mannosides on the host uroepithelial cells. A vaccine to stimulate production of neutralizing antibodies that bind to E. coli FimH is in clinical trials. How would this vaccine prevent UPEC infection?