Homework Answers
Ans.) Option A : Horizontal gene transfer
Explanation: The E. coli O104:H4 acquired Shiga toxin and antibiotic resistance by horizontal gene transfer. Horizontal gene transfer is method by genes present on plasmid DNA is passed to other strains of closely related bacteria by mode of transduction (carried by viruses), Conjugation (transferred via pili) or translation (passed via media).
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The pathogenic strain of Escherichia coli, E. coli O 104: H4, expresses the Shiga toxin and...
1. Which strain of Escherichia coli has been transduced with the gene for the Shiga toxin?...
1. Which strain of Escherichia coli has been transduced with the gene for the Shiga toxin? (A-E) A E പറ B D с 2. Aeromonas hydrophila, the bacterium with this plasmid, was found growing in what organism? 3. The qach and qacEA1 genes provide resistance to what group of disinfectant?? 4. The tetA and tetR genes provide resistance to what type of antibiotics? Aeromones hydrophile plasmid R18 165.906be GC 52.53% 5.. Name the type of horizontal gene transfer diagrammed below....
D. Natural selection acts on the classes of antibiotics and the individual antibiotics evolve. T 8....
D. Natural selection acts on the classes of antibiotics and the individual antibiotics evolve. T 8. How do bacteria that have never been treated by antibiotics acquire genes for antibiotic resistance? (1 pt) These bacteria are naturally resistant to all antibiotics. II. During conjugation, two bacterial cells form a bridge between them and exchange genetic information. This can transfer resistant genes from one cell to another (also known as horizontal gene transfer, plasmid exchange). Bacteria chose to join resistant populations...
The E. coli strain H104:O4 caused the 2011 outbreak in Germany. This strain encodes several virulence...
The E. coli strain H104:O4 caused the 2011 outbreak in Germany. This strain encodes several virulence factors that were acquired through horizontal gene transfer. If you are given the complete genome sequence of this strain, how can you identify the genomic regions containing these horizontally transferred virulence genes?
3. Consider a hypothetical strain of E. coli (strain 401) that contains two mutations that affect...
3. Consider a hypothetical strain of E. coli (strain 401) that contains two mutations that affect tryptophan biosynthesis. The first mutation is a single nucleotide substitution that converts the second tandem Trp codon in region 1 of the Trp leader sequence into a stop codon (see slides 2-7 of Lecture 24 on iLearn) trp structural genes trpE trpD trpC trpB trpA PO Trp Leader Met-Lys-Ala-lle-Phe-Val-Leu-Lys-Gly-Trp-Trp-Arg-Thr Ser- Stop Assume that strain 401 also contains a mutation in the gene for the...
Bacterial cells, such as E. coli, are surrounded by a fluid plasma membrane and enclosed by...
Bacterial cells, such as E. coli, are surrounded by a fluid plasma membrane and enclosed by a rigid cell wall that protects the cell from damage. You will recall that there is no nuclear envelope in prokaryotic cells, but rather a “nucleoid region” with a single chromosome that is continuous (circular), not linear, as in eukaryotes. All of the genes required for basic survival and reproduction are found in the main chromosome. In addition to the main chromosomes, some DNA...
Question 9 infected with nonresistant strains of the tuberculosis bacterium, antibiotics can relieve symptoms in a...
Question 9 infected with nonresistant strains of the tuberculosis bacterium, antibiotics can relieve symptoms in a few weeks. However, it takes much w could this result in the evolution of drug-resistant pathogens? much the terms in the left column to the appropriate blanks in the sentences on the right. Not all terms will be used. Reset He proteins If treatment with antibiotics is stopped and pathogenic bacteria are still present, it may obtain genetic basis for their persistency The natural...
2. You have identified a mutant E. coli strain that cannot synthesize histidine (His). To determine...
2. You have identified a mutant E. coli strain that cannot synthesize histidine (His). To determine the location of the his mutation on the E. coli chromosome, you perform interrupted mating experiments with 5 different Hfr strains. The following chart shows the time of entry (minutes, in parentheses) of the wild-type alleles of the first 5 markers (mutant genes) into the His strain. + + Hfr A. Hfr B Hfr CH Hfr D. Hfr E- his (3) cys (11)- arg...
Question 58 1 pts Name the tick borne disease caused by Ricketsii that acutely features a...
Question 58 1 pts Name the tick borne disease caused by Ricketsii that acutely features a speckled rash on the wrists and ankles and may lead to long term damage due to intracellular pathogenic bacteria destroying epithelial cells of the blood vessels Name the tick borne disease caused by Ricketsii that acutely features a speckled rash on the wrists and ankles and may lead to long term damage due to intracellular pathogenic bacteria destroying epithelial cells of the blood vessels...
2 What is the minimum genotype of a recombinant cell that grew on minimal media supplemented...
2 What is the minimum genotype of a recombinant cell that grew on minimal media supplemented with arginine, methionine and the antibiotic tetracycline but lacking the essential amino acid isoleucine 3 How would you determine if your recombinant cells had also acquired the tryptophan gene from the Hart Complete the following table indicating which of the organisms (F and/or Hfr used in this experiment would be expected to grow on the given media. Unless specified, MMD is just minimal medium...
Need help filling in the chart and answering the questions that go along with it. I...
Need help filling in the chart and answering the questions
that go along with it. I have added the procedure and the
instructions as well as the "results" that are supposed to be used
to fill in the chart. Thank you!
We were unable to transcribe this imageTABLE 8-1 Cast of Characters and a Legend of Abbreviations Name Symbol Function in This Experiment Green fluorescent protein GFP It serves as an indicator of successful transformation and gene transcription expression in...
1. Which strain of Escherichia coli has been transduced with the gene for the Shiga toxin? (A-E) A E പറ B D с 2. Aeromonas hydrophila, the bacterium with this plasmid, was found growing in what organism? 3. The qach and qacEA1 genes provide resistance to what group of disinfectant?? 4. The tetA and tetR genes provide resistance to what type of antibiotics? Aeromones hydrophile plasmid R18 165.906be GC 52.53% 5.. Name the type of horizontal gene transfer diagrammed below....
D. Natural selection acts on the classes of antibiotics and the individual antibiotics evolve. T 8. How do bacteria that have never been treated by antibiotics acquire genes for antibiotic resistance? (1 pt) These bacteria are naturally resistant to all antibiotics. II. During conjugation, two bacterial cells form a bridge between them and exchange genetic information. This can transfer resistant genes from one cell to another (also known as horizontal gene transfer, plasmid exchange). Bacteria chose to join resistant populations...
3. Consider a hypothetical strain of E. coli (strain 401) that contains two mutations that affect tryptophan biosynthesis. The first mutation is a single nucleotide substitution that converts the second tandem Trp codon in region 1 of the Trp leader sequence into a stop codon (see slides 2-7 of Lecture 24 on iLearn) trp structural genes trpE trpD trpC trpB trpA PO Trp Leader Met-Lys-Ala-lle-Phe-Val-Leu-Lys-Gly-Trp-Trp-Arg-Thr Ser- Stop Assume that strain 401 also contains a mutation in the gene for the...
Bacterial cells, such as E. coli, are surrounded by a fluid plasma membrane and enclosed by a rigid cell wall that protects the cell from damage. You will recall that there is no nuclear envelope in prokaryotic cells, but rather a “nucleoid region” with a single chromosome that is continuous (circular), not linear, as in eukaryotes. All of the genes required for basic survival and reproduction are found in the main chromosome. In addition to the main chromosomes, some DNA...
Question 9 infected with nonresistant strains of the tuberculosis bacterium, antibiotics can relieve symptoms in a few weeks. However, it takes much w could this result in the evolution of drug-resistant pathogens? much the terms in the left column to the appropriate blanks in the sentences on the right. Not all terms will be used. Reset He proteins If treatment with antibiotics is stopped and pathogenic bacteria are still present, it may obtain genetic basis for their persistency The natural...
2. You have identified a mutant E. coli strain that cannot synthesize histidine (His). To determine the location of the his mutation on the E. coli chromosome, you perform interrupted mating experiments with 5 different Hfr strains. The following chart shows the time of entry (minutes, in parentheses) of the wild-type alleles of the first 5 markers (mutant genes) into the His strain. + + Hfr A. Hfr B Hfr CH Hfr D. Hfr E- his (3) cys (11)- arg...
Question 58 1 pts Name the tick borne disease caused by Ricketsii that acutely features a speckled rash on the wrists and ankles and may lead to long term damage due to intracellular pathogenic bacteria destroying epithelial cells of the blood vessels Name the tick borne disease caused by Ricketsii that acutely features a speckled rash on the wrists and ankles and may lead to long term damage due to intracellular pathogenic bacteria destroying epithelial cells of the blood vessels...
2 What is the minimum genotype of a recombinant cell that grew on minimal media supplemented with arginine, methionine and the antibiotic tetracycline but lacking the essential amino acid isoleucine 3 How would you determine if your recombinant cells had also acquired the tryptophan gene from the Hart Complete the following table indicating which of the organisms (F and/or Hfr used in this experiment would be expected to grow on the given media. Unless specified, MMD is just minimal medium...
Need help filling in the chart and answering the questions
that go along with it. I have added the procedure and the
instructions as well as the "results" that are supposed to be used
to fill in the chart. Thank you!
We were unable to transcribe this imageTABLE 8-1 Cast of Characters and a Legend of Abbreviations Name Symbol Function in This Experiment Green fluorescent protein GFP It serves as an indicator of successful transformation and gene transcription expression in...
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