Question

help I dont know which of these two is correct

Incorrect Question 7 0/0.5 pts (Multiple Choice) There are many similarities and differences between prokaryotic and eukaryotic DNA. This changes the way in which genes of interest are obtained and used for recombinant DNA/transformation experiments. Choose the correct statement below. No answer text provided No answer test provided For prokaryotes, the pene of interest can be cut directly out of the penome and inserted into the bland For prokaryotes scientists need to collect mature mRNA that encodes the gene of interest for these For eukaryotes the sense of interest can be directly out and used from the DNA pename Question 8 Not yet graded/2.5 pts

Answer 1

Answer: There are many similarities and differences between the DNA of prokaryotes and eukaryotes. One of the differences is presence of introns in eukaryotic DNA while absence in prokaryotic DNA. Introns are the non- coding regions of eukaryotic DNA which are spliced out (removed) by splicing during post transcriptional modifications.

So, for eukaryotes the gene of interest can't be cut directly from DNA rather the mature mRNA is required for cutiing out the gene of interest. A mature mRNA is produced after removal of introns by splicing and consists of exons, i.e., coding regions only. From this mRNA complementary cDNA is synthesized and used as gene of interest for transformation experiments. While as there are no introns present in prokaryotes, the gene of interest hence can be cut directly from DNA and used for experiments in transformation.

So, option 'A' is correct, i.e., for prokaryotes, the gene of interest can be cut directly out of the genome and inserted into the plasmid.

Both the other option are wrong.