A gram stain of bacterium A grown 72 hours shows both purple and pink stained cells....
A gram stain of bacterium A grown 72 hours shows both purple and pink stained cells. Additional analysis shows that bacterium A is NOT contaminated. Explain these results.
Homework Answers
Gram stain of bacterium A grown for 72 hours shows the presence of both purple and pink stained cells because after 72 hours of growth the bacterial cell culture becomes old due to which the cell wall of Gram positive bacteria is weakened. As a result, the thick peptidoglycan of the Gram positive bacterial cell wall begins to leak out due to which the Gram staining property of a cell changes from Gram positive to Gram negative, as the cell lacks peptidoglycan. Hence, after 72 hours few cells appear pink in colour as they retain the colour of counter stain safranine.
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A gram stain of bacterium A grown 72 hours shows both purple and
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Salmonella is associated with the handling of contaminated beef True False Escherichia coli is a red-pigmented organism. True O False The results of the MR-VP test can be directly read from the test without the addition of any other chemicals. True False Which medium contains a pH indicator to show pH change caused by fermentation? Glucose Broth Lactose Broth SIM Lactose and...
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A Gram stain of unknown bacterium A grown for 24 hours show purple cells. Is bacterium A Gram positive or Gram negative? A gram stain of bacterium A grown for 72 hours shows both purple and pink stained cells. Additional analysis shows that bacterium A is NOT contaminated. Explain these results. You are given 2 nutrient broth tubes each containing a pure culture of one of two Gram positive bacteria. Unfortunately, the labels have washed off and you are asked...
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5. You use the Gram stain to try to differentiate bacteria in a sample. You find that all of the cells are Gram negative, but the teaching assistant insists there are both Gram positive and Gram negative cells present in the sample you are given. You definitely smeared a loop of the sample onto the slide and your fingers are stained a little purple and also a little red from handling the sides of the slide. Give a reason why...
Questions 1 Often, upon doing a negative pom doing a negative stain of unencapsulated Gram-negative cells, a small halo will be seen around the cells. Why is this the case? What external structure of Gram-negative cell will acude the negative stain? 2 What are the functions of a bacterial capsule? 3 Does a species produce a capsule under all conditions of cultivation? Explain. 4 Why is the size of a bacterium more accurate for a negative stain than a direct...
Microbiology
ONLY answer
1
2
3
4
5
6
7
8
9
10
11
12
13
14
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Salmonella is associated with the handling of contaminated beef True False Escherichia coli is a red-pigmented organism. True O False The results of the MR-VP test can be directly read from the test without the addition of any other chemicals. True False Which medium contains a pH indicator to show pH change caused by fermentation? Glucose Broth Lactose Broth SIM Lactose and...
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