Homework Answers
2. Kovacs reagent is added in the indole test to identify Enterobacteria members. The Enterobacteria members gives positive test for kovacs reagant. Enterobacteria members are having the ability to decompose the tryptophan to indole. Tryptophan is reductively deaminated to produce indole, pyruvic acid,ammonium and energy. Indole unites with kovacs reagent solution turns yellow to red. Kovacs reagent contains Hcl and p- dimethylaminobenzaldehyde in amyl alchohol. Amyl alchohol is not soluble in water and red colour will form in oily layer later at the top.
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18. Laboratory Report: Questions 1. Why might hydrolysis reactions be important to identifying microbes from 1)...
Please answer 1-4 1 Discuss some difficulties one might encounter in differentiating bacteria on the basis...
Please answer 1-4
1 Discuss some difficulties one might encounter in differentiating bacteria on the basis of physiological tests 2. Hopefully, you noticed all the reminders to incubate the test tubes with loose Gaps. Why is this important? 3. How would you determine whether an organism can ferment other carbohydrates, such as mannitol? 4. What enzymes are involved in the following reactions: Urea hydrolysis- Hydrogen sulfide production- Casein hydrolysis- Indole production- Catalase-
1. Why is it important to identify microbes disease process? 2. What is a bacterial culture?...
1. Why is it important to identify microbes disease process? 2. What is a bacterial culture? Why do we perform this procedure? 3. How does the number of disease microbes at the site of entry relate to the process of infection? 4. List the tools for identifying microbes and example of each. 5. A surgical site infection may start as an abscess. What exactly is an abscess? 6. Why are bacteria the focus of study in disease microbiology? 7. You...
369 Name Locker Number Date Plesse print: last name first REPORT SHEET (continued): Experiment 26 Hydrolysis of a D...
369 Name Locker Number Date Plesse print: last name first REPORT SHEET (continued): Experiment 26 Hydrolysis of a Disaccharide 5. Does sucrose give a positive Benedict test? Why or why not? After hydrolysis, did sucrose give a positive Benedict test? Explain your answer. 6. Bial Test What color indicates a positive test for a pentose sugar? Record your observations +or - for each carbohydrate in the table below: 1. xylose 3. fructose 5. starch 2. glucose 6. control 4. sucrose...
Can someone answer these questions please!? Need help!!!! 1. Compare the optimum pH results obtained ...
Can someone answer these questions please!? Need help!!!! 1. Compare the optimum pH results obtained for amylase and pepsin with those predicted in the Pre-Laboratory Assignment. Discuss any possible discrepancies between the predicted and experimental results. 2. What was the purpose of including control samples containing only substrate and buffer (test tubes 1−4 for each enzyme) in this study? Did any of these mixtures show any reaction? 3. Protein digestion involves the hydrolysis of peptide linkages and ...
22 > Laboratory Review Questions and Problems af summarize 1. After discussing the results of all...
22 > Laboratory Review Questions and Problems af summarize 1. After discussing the results of all four experiments in cussing the results of all four experiments in Exercise A with your classmates, briefly summare rate concentration your conclusion onclusions on the effects of temperature, plengyme concentration, and substrate concentras on the action of enzymes. 2. What important consequences do these properties of enzymes (from question 1) have to lism of cells and organisms? 3. Define enzyme denaturation in terms of...
Laboratory Instructor STUDENT REPORT A. Data Sheet EXPERIMENT 28 Aspartame 1. Thin-layer chromatography Distance traveled by...
Laboratory Instructor STUDENT REPORT A. Data Sheet EXPERIMENT 28 Aspartame 1. Thin-layer chromatography Distance traveled by solvent (1) Substance Distance teweled DR value Calculations: 2. KMnO, test Observations 10 min. 15 min. Reaction? 5 min. Substance 1092 330 Exploring Chemistry 3. Cari nitrate test B. Reflective Exercises and phenylalanine columns on the 1. How many spots should be seen for each of the aspartic acid and price chromatogram? Why? 2. Referring to the chromatogram, what did you here and what...
QUESTIONS Answer the following questions in the data section of your lab report: 1. Use the...
QUESTIONS Answer the following questions in the data section of your lab report: 1. Use the experience you gained from doing the experiment to answer the following: A student tested an unknown mixture that might contain any of the ions tested in this ex- periment and made the following observations: a. On the addition of 6M HCI, the solution remained colorless and no bubbles were observed. b. When 0.1M BaCl2 was added to the acidified unknown, a white precipitate was...
please answer questions 1 through 6 with the information provided. Enzyme Specificity Are Sucrase and Lactase...
please answer questions 1 through 6 with the
information provided.
Enzyme Specificity Are Sucrase and Lactase specific to Sucrose and Lactose? I: Experimental Procedure 1. Add 0.3 g of sucrose to three different test tubes. Make sure to remember to label them 1 to 3. 2. Add 5 mL of Dl water to test tube number 1. Add 5 mL of sucrase to test tube 2. Add 5 ml of lactase to test tube 3. 3. Add 0.3 g of...
Name l_ent 11 Lab Report-ENZYMES: HYDROLYSIS OF STARCH BY AMYLASE Partner Section l113 Station # ...
Name l_ent 11 Lab Report-ENZYMES: HYDROLYSIS OF STARCH BY AMYLASE Partner Section l113 Station # PART B. Hydrolysis of starch by increasing amounts of extract Table 1. Absorbance at 590 nm after 5 min incubation of starcn % of starch remaining ps of | Absorbance at 590 | oextract nm after incubation after 5 min incubation 100 0 .312 laa 3 3 5 absorbance of tube O (no enzyme) reflects the total amount of starch initially nt, so the %...
please fill out chart and answer applicable questions attached for thumbs up Part 1: Characterization of...
please fill out chart and answer applicable questions attached
for thumbs up
Part 1: Characterization of carbohydrates with and without digestion by enzymes (You can refer to the complete Lab Procedure which is posted separately for more information about this part of the lab.) You are given a set of samples: starch, sucrose, and glucose. The samples are tested before and after different enzymes are added. After the enzymes are added, the samples are incubated in a 37°C water bath...
Please answer 1-4
1 Discuss some difficulties one might encounter in differentiating bacteria on the basis of physiological tests 2. Hopefully, you noticed all the reminders to incubate the test tubes with loose Gaps. Why is this important? 3. How would you determine whether an organism can ferment other carbohydrates, such as mannitol? 4. What enzymes are involved in the following reactions: Urea hydrolysis- Hydrogen sulfide production- Casein hydrolysis- Indole production- Catalase-
369 Name Locker Number Date Plesse print: last name first REPORT SHEET (continued): Experiment 26 Hydrolysis of a Disaccharide 5. Does sucrose give a positive Benedict test? Why or why not? After hydrolysis, did sucrose give a positive Benedict test? Explain your answer. 6. Bial Test What color indicates a positive test for a pentose sugar? Record your observations +or - for each carbohydrate in the table below: 1. xylose 3. fructose 5. starch 2. glucose 6. control 4. sucrose...
22 > Laboratory Review Questions and Problems af summarize 1. After discussing the results of all four experiments in cussing the results of all four experiments in Exercise A with your classmates, briefly summare rate concentration your conclusion onclusions on the effects of temperature, plengyme concentration, and substrate concentras on the action of enzymes. 2. What important consequences do these properties of enzymes (from question 1) have to lism of cells and organisms? 3. Define enzyme denaturation in terms of...
Laboratory Instructor STUDENT REPORT A. Data Sheet EXPERIMENT 28 Aspartame 1. Thin-layer chromatography Distance traveled by solvent (1) Substance Distance teweled DR value Calculations: 2. KMnO, test Observations 10 min. 15 min. Reaction? 5 min. Substance 1092 330 Exploring Chemistry 3. Cari nitrate test B. Reflective Exercises and phenylalanine columns on the 1. How many spots should be seen for each of the aspartic acid and price chromatogram? Why? 2. Referring to the chromatogram, what did you here and what...
QUESTIONS Answer the following questions in the data section of your lab report: 1. Use the experience you gained from doing the experiment to answer the following: A student tested an unknown mixture that might contain any of the ions tested in this ex- periment and made the following observations: a. On the addition of 6M HCI, the solution remained colorless and no bubbles were observed. b. When 0.1M BaCl2 was added to the acidified unknown, a white precipitate was...
please answer questions 1 through 6 with the
information provided.
Enzyme Specificity Are Sucrase and Lactase specific to Sucrose and Lactose? I: Experimental Procedure 1. Add 0.3 g of sucrose to three different test tubes. Make sure to remember to label them 1 to 3. 2. Add 5 mL of Dl water to test tube number 1. Add 5 mL of sucrase to test tube 2. Add 5 ml of lactase to test tube 3. 3. Add 0.3 g of...
Name l_ent 11 Lab Report-ENZYMES: HYDROLYSIS OF STARCH BY AMYLASE Partner Section l113 Station # PART B. Hydrolysis of starch by increasing amounts of extract Table 1. Absorbance at 590 nm after 5 min incubation of starcn % of starch remaining ps of | Absorbance at 590 | oextract nm after incubation after 5 min incubation 100 0 .312 laa 3 3 5 absorbance of tube O (no enzyme) reflects the total amount of starch initially nt, so the %...
please fill out chart and answer applicable questions attached
for thumbs up
Part 1: Characterization of carbohydrates with and without digestion by enzymes (You can refer to the complete Lab Procedure which is posted separately for more information about this part of the lab.) You are given a set of samples: starch, sucrose, and glucose. The samples are tested before and after different enzymes are added. After the enzymes are added, the samples are incubated in a 37°C water bath...
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