Question

3. Record the class data below for the number of plants of each genotype: +/+ ns4/+ _22 (Homozygous Wild-Type) (Heterozygous) (Homozygous Mutant) Musings 4. Perform a Chi-squared test on the results above to test: Hypothesis- the observed numbers of cach genotype do not deviate significantly from a 1:2:1 genotypic ratio. a. First, calculate the expected number of each genotype based on the total number of plants tested. Report the values to two places after the decimal. (Yes, the expected numbers can include fractions of a plant). Total number of plants tested in the class: Expected: +/+ rus 4/+ (=Total x 0.25) (=Total x 0.50) (=Total x 0.25) rus4/rus4 b. The calculation of the chi-squared for three categories is as below: (observed expected) + (observed - expected) + (observed - expected) expected expected expected for: [ +/+] [rus/+] [ rus4/rus] c. If the y value is greater than 5.99, we can reject our hypothesis, meaning, the class data does not fit the expected 1:2:1 ratio of genotypes. Does your statistical analysis Support or Reject the hypothesis?

Answer 1

A Toto number d plants = 37. Expected +/+ = 0.25X37 = 9.25 7034/7 = 0-5x37 = 18.5 Tus4/0034 = 0.25x37 = 9.25 obsened (0) Expected (E) o-E 9.25 YUS/4 22 18.5 3.5 144/0004 9.25 1:25 6. EE 0.547 0.662 0.168 x ²21 377 Total 32 < 5.99, Accept the hypothesis. . It is 1:2:1 ratio.