Question

I need help with a,b,c, and d please and explain how you got it please.

1) Answer the following questions based on the image above (10) A) What is the question being asked in figure parts A and B above (2)? B) What is the result (answer) to the question based on data shown in part A and B? Justify your answer by highlighting the data supporting your response (2). C) Propose a possible mechanism by which this protein could produce the results indicated above. Make sure to consider the mechanism of action of both compounds (2) D) Which of the mechanism seem more likely? Design an experiment to test this hypothesis. Show your expected data generated from this experiment (4). SKOV3: 48 h Cisplatin B SKOV3:48 h Paclitaxel a. C-N/S X Survival Survival 0 1.56 3.125 6.25 Concentration (M) 0.625 1.25 25 Concentration (M - -- a • -NV qua đ --- 0 SKOV3-NV, SKOV3-C1, and SKOV3-C7 cells were treated with 0-12. 5M cisplatin (a) or 0-5 nM paclitaxel (b) for 48 h, at which time the cells were subjected to MTS assay to measure viability. Cl and C7 were transfected with an YFG (protein) over expressing construct, while NV was transfected with only control construct. Results are displayed as percent survival of vehicle treated cells. Error bars represent the

Answer 1

A)ANSWER what is the effect of drug cisplatin and paclitaxel on the SKOV3-NV, SKOV3-C1, And SKOV3-C7 cells under different concentrations in diagram A and B?

B)ANSWER Paclitaxel drug is more effective than cisplatin in cancer. In comparison with SKOV3-NV, SKOV3-C1, and SKOV3-C7 cells skov3-NV work as control which is more affecting from paclitaxel than cisplatin drug.when we input our gene of interest with C1 and C7 cells C1 cells show better results of the drug against cancer.

C)ANSWER As such we observe the given A and B diagram we conclude that both drugs are helpful in cancer-preventing in the gene of interest. we use paclitaxel in different cancer in a lower amount.

D)ANSWER we can design a hypothesis in which this type of drug is helpful in preventing cancer. we set a target oncogene in which injecting the drug and identify the effects. If our experiment will successful then we use these drugs in oncology study.