Question

I need help with 1a and 1b please. Can you explain how you got the answer to. It is for cellular and molecular biology. Ignore the blue dot on figure b.

SKOV3-NV, SKOV3-C1, and SKOV3-C7 cells were treated with 0-12.5 M cisplatin (a) or 0-5 nM paclitaxel (b) for 48 h, at which time the cells were subjected to MTS assay to measure viability. C1 and C7 were transfected with an YFG (protein) over expressing construct, while NV was transfected with only control construct. Results are displayed as percent survival of vehicle treated cells. Error bars represent the standard deviation of biological replicates. *p<.05, **p<.005, N/S = not significant SKOV3:48 h cisplatin B SKOV3:48 h Paclitaxel 07-N/S X Survival * Survival o 1.56 3.125 6.25 5 Concentration (M) 12. 0 0.625 1.25 2. 5 Concentration (M) 5 -NV ...... C1 --- C7 — NV .... CI ---C7 1) Answer the following questions based on the image above (10) A) What is the question being asked in figure parts A and B above (2)? B) What is the result (answer) to the question based on data shown in part A and B? Justify your answer by highlighting the data supporting your response (2). DE

Answer 1

A) In the figure of part A and B, the survival of three cells in the presence of thwo drugs cisplatin and placiltaxel were given . So the from the graph, the question would be reagarding the difference in the survival of the cells due to the variation in the transfection of a YGF protein among the cells.

B) According to the data given, the cell (NV), transfected with control construct significantly resulted in decrease in the survival. Whereas, the other two cells, (C1 and C7), which were transfected with overexpressed contruct (YGF), the percentage survival was restored significantly, especially at high concentrations of the drugs.