No in a closed system or batch culture the products are not removed continously. Therefore, infinitely increasing the substrate concentration will not help as the enzyme would become saturated. Moreover, this would still work if the system was not closed.
Can you infinitely infrease the substrate concentration to increase enzyme activity in a closed system such...
The rate of an enzyme-catalyzed reaction initially increases with an increase in the substrate concentration, but eventually reaches a maximum value, even though the concentration of substrate continues to increase. Which of the following best explains why? O As substrate concentration increases, the substrates preferentially bind with each other instead of the active site of the enzyme, and no additional catalysis occurs. As substrate concentration increases, the active sites of all the enzyme molecules become occupied with substrate molecules, and...
Review your knowledge of how enzyme activity is affected by substrate concentration by selecting the correct statements concerning enzyme activity at low substrate concentrations. Contact between enzyme and substrate is rare Velocity of the reaction increases Enzyme makes product rapidly Enzyme makes product slowly Enzyme is saturated with substrate
Explain the mechanism of how substrate concentration affects enzyme activity and how this mechanism yields the expected summary curve
Enzyme activity will be high when substrate concentration is equal to KM b. below KM c. above KM d. none of the above Metalloproteases mostly use the element Cu b. Fe c. Zn d. Mn
Annabeth is testing the effect of substrate concentration on enzyme activity using cellobiase and p-nitrophenyl glucopyranoside. After she adds STOP solution to her reactions and measures the absorbances, she discovers that one sample has an absorbance that is beyond the range of her standard curve. How can Annabeth determine the concentration of product for this sample?
List and describe the factors that can affect enzymatic reactions. a. Substrate Concentration b. Enzyme Concentration c. pH d. Temperature e. Cofactors f. Inhibitors (I have them listed but do not understand their affects)
1) 2) If the total enzyme concentration was 9 nmol/Lnmol/L, how many molecules of substrate can a molecule of enzyme process in each minute? Express your answer to three significant figures. 3) The kcat for neuraminidase at pH=6.15pH=6.15, 37 ∘C∘C is 26.8 s−1s−1. Calculate KMKM for the hydrolysis of sialic acid. Express your answer with the appropriate units. An enzyme that follows Michaelis-Menten kinetics has a Ky value of 6.00 uM and a keat value of 176 s-1. At an...
If the substrate concentration is varied with a fixed concentration of enzyme, it is observed that at low substrate concentrations that overall order while at high substrate concentrations, the reaction will be overall order. Select one: a. first; second b. first; third order c. first; zero d. second; second e. second; pseudo first order
me cel g tha SUBSTRATE Enzyme A Inactive intermediate 1 e con e con Enzyme B e actis e con Inactive intermediate 2 le con Enzyme C know Active product Given the cellular negative feedback example shown, select all of the following that would result from taking a drug that inhibits the activity of Enzyme C. Check all that apply. The concentration of intermediate 2 would increase. The concentration of intermediate 1 would increase. The activity of Enzyme A would...
COST-LAB QUESTIONS: 1. What happens to enzyme activity as the enzyme concentration is increased? 2. Based on your results, what is the optimum pH for lactase? 3. Do all enzymes in the body have the same optimum pH? Why or why not? 4. What happens to enzyme activity as the substrate concentration is increased? Explain why this occurs.