Question

True/False

6. a)A tryptophan auxotrophic strain (trp¯) of bacteria will be able to grow on a minimal medium agar that has been supplemented with the amino acid histidine.

b). Hfr strains result from the insertion and recombination of the F factor plasmid into the bacterial chromosome.

c). In generalized transduction, any gene or set of genes on the chromosome may be transferred from the donor bacterium to the recipient bacterium.

  d). The order of genes on a bacterial chromosome can be determined by using transduction.

Answer 1

A. False

First statement is incorrect because the given bacterial strain is not able to synthesise tryptophan. So it will not grow on minimal media Agar supplemented with histidine. Instead the media plate should be supplemented with tryptophan.

B. True

The insertion of fertility factor plasmid into bacterial chromosome results in the formation of hfr strain and the DNA present in hfr is called as episome.

C. False

In generalized transduction, any set of genes can be transferred from virus to the recipient bacterium.

D. True

The order of the genes on a chromosome can be determined by cotransduction frequency between different genes.

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