Question

QUESTION 35 Tetracycline is an antibiotic used to treat bacterial infections in humans because it prevents the growth and spread of bacteria. This drug is known to bind strongly and specifically to the A site in the 30S ribosomal subunit. Based on this information you would expect that: a. tetracycline does not affect translation in eukaryotic cells. b. tetracycline inhibits RNA splicing in prokarytes. c. tetracycline will prevent the small ribosomal subunit from binding to the Shine-Dalgarno sequence. d. tetracycline prevents the growth of bacteria by blocking the binding of charged-tRNA molecules to the complex of the small ribosomal subunit + mRNA. e. tetracycline prevents the fMet-tRNA's anticodon from base-pairing with the AUG codon.

Answer 1

the ribosome in the prokaryotes are 70S, the smaller subunit is 30S and the larger subunit is 50S and in the eukaryotes the ribosomes are 80S, the smaller subunit is 40S and the larger subunit is 60S, the tetracycline which blocks the translation by binding to the small subunit 30S subunit of the ribosome cannot bind to th 40S subunit, the tetracycline binds to the 30S subunit and prevents its binding to the mRNA, usually 30S subunit first binds to the mRNA at the ribosome binding site. the charged tRNA binds to the mRNA only after the association of the 30S subunit to the mRNA.

tetracycline inhibits translation in prokaryotes

Prokaryotes do not process their mRNA.

so the answer is  

a) tetracycline does not affect translation in eukaryotes.

c) tetracycline will prevent the small ribosomal subunit from binding to the shine-Dalgarno sequence.

( Shine-Dalgarno sequence is the ribosome binding site in prokaryotes)