In an Ames Test used for two Salmonella strains TA-98 and TA-100, which chemical compound is...
In an Ames Test used for two Salmonella strains TA-98 and TA-100, which chemical compound is mutagenetic?
a) Dimethyl Sulfoxide (DMSO)
b) 2-aminoflourene (2-AF)
Homework Answers
Answer - 2-aminoflourene ( 2-AF) is used as chemical mutagenic agent. ( It is a derivative of fluorene, which has mutagenic property and used as biochemical tool in study of carcinogenesis. )
( While DMSO is used to decrease pain, speed healing of wounds , burns, muscle and skeletal injuries. )
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In an Ames Test used for two Salmonella strains TA-98
and TA-100, which chemical compound is...
You just found a new compound “ABC” to be carcinogenic via the Ames test using His-Salmonella....
You just found a new compound “ABC” to be carcinogenic via the Ames test using His-Salmonella. Which of the experimental plates listed below will have the most colonies? His-Salmonella, rat liver enzymes His-Salmonella, rat liver enzymes, compound “ABC” His-Salmonella, compound “ABC” What test can you do to confirm that “ABC is carcinogenic?
1.The His- phenotype of an Salmonella strain is caused by a single-nucleotide deletion. For this strain,...
1.The His- phenotype of an Salmonella strain is caused by a single-nucleotide deletion. For this strain, we can expect to find His+ revertants after treatment with: Group of answer choices A.X-rays B. Acridine orange (intercalating agent) C. Nitrous acid (deaminating agent) D. This mutation is very unlikely to be reverted 2) The His- phenotype of an Salmonella strain is caused by a transition mutation. For this strain, we can expect to find His+ revertants after treatment with: Group of answer...
Below are results of the Ames Test that was conducted to determine whether a compound called...
Below
are results of the Ames Test that was conducted to determine
whether a compound called sodium azide could be useful for causing
detrimental mutations in bacteria, thus preventing bacterial growth
on surgical tools. To test the mutagenicity of sodium azide, the
Ames test was carried out. The bacterial species Salmonella
typhimurium requires Histidine to grow and was plated on minimum
growth plates lacking histidine. Bacteria plated on the control
plate had only buffer added, while bacteria on the treatment...
The estimated spontaneous mutation rate for Salmonella strain A is: _____71____________________. The estimated spontaneous mutation rate...
The estimated spontaneous mutation rate for Salmonella
strain A is: _____71____________________.
The estimated spontaneous mutation rate for Salmonella
strain B is: _______13__________________.
The estimated spontaneous mutation rate for the E.
coli strain is: ___________29______________.
Why might Salmonella strain A and B and the E.
coli strain have different spontaneous mutation rates? Think
about which strain may have a better DNA repair system and how
membrane permeability can affect mutation rates.
Based on the number of colonies per plate, when do...
The researchers grow and maintain the Ames test bacteria in liquid media containing histidine. They then...
The researchers grow and maintain the Ames test bacteria in liquid media containing histidine. They then prepare plates (semi-solid media) with media that lacks histidine. They also prepare tubes of media that is between the semi-solid and liquid media (soft agar) that contains trace amounts of histidine. They inoculate tubes of the soft agar with the mutant Ames test bacteria and add one of the following - 100 l of (1) chemical A in a solvent, (2) chemical B in...
QUESTION 11 does not require supplemental histidine). True or False: Using the Ames test, a compound...
QUESTION 11 does not require supplemental histidine). True or False: Using the Ames test, a compound is determined to be mutagenic if it causes a His bacterial strain (requires histidine in the growth medium) to become His O True False QUESTION 12 Xeroderma pigmentosa (XP): is caused by a defect in the mismatch repair system. O typically results in base substitution mutations. can be caused by defects in any of a number of different genes that have to do with...
NGLES FOR ANY POLYGON-180 (N-2 IN- Name: Which of the follwing is used for chemical symbols...
NGLES FOR ANY POLYGON-180 (N-2 IN- Name: Which of the follwing is used for chemical symbols today? d. numbers 44. С. letters 一 a· drawings b. icons 45. The chemical formula of a compo and does NOT indicate the ー identity of the elements in the compound a. b. how elements are joined in the compound c. the composition of the compound d. relative proportions of the elements in the compound 46. Which substance has a chemical symbol that is...
which of the following reagents might serve as the basis for a simple chemical test that...
which of the following reagents might serve as the basis for a
simple chemical test that would distinguish between 2-butane and
2-butanol ?
Is IR considered "simple"
4 d. 5 e. 6 22. Whic h of the following reagents might serve as the basis for a simple chemical test that would distinguish between 2-Butene and 2-Butanol? a. CrO3 in H2SO0A4 es b. I.R. spectrum Yes d. e. two of the above all of the above
rated C-H Single bonds O-HV b) Name the two major chemical tests you would perform on...
rated C-H Single bonds O-HV b) Name the two major chemical tests you would perform on the infrared spectrum pictured above. Test 1_ Jones Test (.5 pt) Test 2_ Lucas Test (.5 pt) 2. What test (name of the test and reagents included) could be used to distinguish between each pair of compounds? Explain the expected observations for each compound (2 pts each): 1) 1-butanol and 2-butanol 2) butanal and 2-butanone 3) propanal and ethyl acetate 4) ethyl acetate and...
Which of the following reagents might serve as the basis for a simple chemical test that...
Which of the following reagents might serve as the basis for a
simple chemical test that would distinguish between the compounds
in the picture. Please explain.
Practice Problems 11 and12-2 IC bility Mode Ward PLE HOME INSERT DESIGN PAGE LAYOUT Karen Herrera Garcia REFERENCES MAILINGS REVIEW VIEW cut Find Times New Ro 12 A A AaBbC AaBbC I AaBbC AaBboc AaBbccD AaBbccDo AaBbccC Rep EE Copy Past B I U x, xa A A EEE E Heading 1 Heading 2...
Below
are results of the Ames Test that was conducted to determine
whether a compound called sodium azide could be useful for causing
detrimental mutations in bacteria, thus preventing bacterial growth
on surgical tools. To test the mutagenicity of sodium azide, the
Ames test was carried out. The bacterial species Salmonella
typhimurium requires Histidine to grow and was plated on minimum
growth plates lacking histidine. Bacteria plated on the control
plate had only buffer added, while bacteria on the treatment...
The estimated spontaneous mutation rate for Salmonella
strain A is: _____71____________________.
The estimated spontaneous mutation rate for Salmonella
strain B is: _______13__________________.
The estimated spontaneous mutation rate for the E.
coli strain is: ___________29______________.
Why might Salmonella strain A and B and the E.
coli strain have different spontaneous mutation rates? Think
about which strain may have a better DNA repair system and how
membrane permeability can affect mutation rates.
Based on the number of colonies per plate, when do...
QUESTION 11 does not require supplemental histidine). True or False: Using the Ames test, a compound is determined to be mutagenic if it causes a His bacterial strain (requires histidine in the growth medium) to become His O True False QUESTION 12 Xeroderma pigmentosa (XP): is caused by a defect in the mismatch repair system. O typically results in base substitution mutations. can be caused by defects in any of a number of different genes that have to do with...
NGLES FOR ANY POLYGON-180 (N-2 IN- Name: Which of the follwing is used for chemical symbols today? d. numbers 44. С. letters 一 a· drawings b. icons 45. The chemical formula of a compo and does NOT indicate the ー identity of the elements in the compound a. b. how elements are joined in the compound c. the composition of the compound d. relative proportions of the elements in the compound 46. Which substance has a chemical symbol that is...
which of the following reagents might serve as the basis for a
simple chemical test that would distinguish between 2-butane and
2-butanol ?
Is IR considered "simple"
4 d. 5 e. 6 22. Whic h of the following reagents might serve as the basis for a simple chemical test that would distinguish between 2-Butene and 2-Butanol? a. CrO3 in H2SO0A4 es b. I.R. spectrum Yes d. e. two of the above all of the above
rated C-H Single bonds O-HV b) Name the two major chemical tests you would perform on the infrared spectrum pictured above. Test 1_ Jones Test (.5 pt) Test 2_ Lucas Test (.5 pt) 2. What test (name of the test and reagents included) could be used to distinguish between each pair of compounds? Explain the expected observations for each compound (2 pts each): 1) 1-butanol and 2-butanol 2) butanal and 2-butanone 3) propanal and ethyl acetate 4) ethyl acetate and...
Which of the following reagents might serve as the basis for a
simple chemical test that would distinguish between the compounds
in the picture. Please explain.
Practice Problems 11 and12-2 IC bility Mode Ward PLE HOME INSERT DESIGN PAGE LAYOUT Karen Herrera Garcia REFERENCES MAILINGS REVIEW VIEW cut Find Times New Ro 12 A A AaBbC AaBbC I AaBbC AaBboc AaBbccD AaBbccDo AaBbccC Rep EE Copy Past B I U x, xa A A EEE E Heading 1 Heading 2...
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