Question

7. Following the action of DNA polymerase during lagging strand synthesis, there remains two 'problems that must be solved by subsequent steps. What are these "problems? 8. Three events will occur to fix the problems listed in 27 above. What are these events? Be sure to list the enzyme responsible for causing the event. 9. Below, draw the resulting lagging strand that would occur after the three oes listed in Q8 have been made. Draw an outline box around any RNA molecules that remain so that it is clear where they are. CTGGTTAGGCTAGCAACC 3 S' CTTGCTAGG 3' GAACGATCC GTA GOCATOCTAGGGTCGCGAGGEN CCCAGOGCTCCGACCM FATCGTTOGS 10. The figure above shows the very wart of replication. Assume that Dra protein has bound to the DraA Box target sites causing the DNA to twist and produce the open DNA structure shown. What are the three proteins/ enzymes that must function at this point to ensure the continued expansion of the open DNA (Replication Bubble)?
Can someone please help with 8, 9, and 10? Thank you!

Answer 1

8. The three events that occur on the lagging strand are
i. Removal of primers by 5'-3' exonuclease activity of DNA polymerase or RNase H

ii. Replacement of primer with deoxyribonucleotides by 5'-3' polymerization activity of DNA polymerase

iii. Joining of two DNA fragments by DNA ligase