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Below is a portion of your hypothetical dichotomous key for one of your unknown bacteria. Your TA has given you approval to s

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One Kligers slant with unknown bacteria inoculated and one control slant should be prepared.

Because while proceeding with the Dichotomous key, we start from the top. We will test for indole results. From the indole result, we may proceed either with Esculin or Glucose. From the result of esculin or glucose, we will proceed with (Kligers or Lactose) OR (Kilgers or Raffinose).

Working with dichotomous key is like you start with a question and with the results you proceed with the next question.

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