In a minimal medium different amino acids remain absent hence the growth rate will be slow as compared to TSB. In presence of glucose organism will take time to produce enzymes due to Glucose Effect. So initially growth will be slow and after the production of enzymes growth will be faster. In a minimal media with glucose organism will show diauxic shift. The growth curve will be similar to classic curve but adaptation time or lag phase will be longer than in TSB.
Assume that the growth curve was done using complex medium like TSB. Predict what the growth...
Assume that the growth curve was done in a medium containing 7.5% NaCl. predict what the growth curve would look like if you grew the bacterium(E-coli) in medium containing 0.9% NaCl.
A bacterium is grown at its optimal growth temperature of 37°C in a chemostat. Describe and explain what would happen to the shape growth curve (what phase, the slope, etc) of the culture if: The chemostat was placed at 27°C. The chemostat at 37°C broke and no longer supplied new nutrients or removed wastes. You took a sample out of the chemostat containing a complex media and placed it into media that was chemically defined, minimal media You took a...
3. Using a diagram show the growth curve for a bacterial organism grown in batch culture. Label the various phases and describe what is happening in each phase. How can we juggle the conditions to create a continuous culture as in a situation where we want a continuous supply of a bioproduct.
17.A viable count of this culture, which represents Staphylococcus aureus, was done after 120 minutes. How many CFUs are expected if you plated 0.2 mL of a 104 dilution? (Hint: Consider the average number of cells per CFU) 18. At 65 minutes, the cells were transferred to a new medium in which the growth rate (H) was three times lower. What is the expected number of cells after 60 minutes of growth following the transfer? 19.By what factor did the...
QUESTION 1 Given doubling number and limits predict growth curve. Label all phases of the growth curve Athrough D. State what the X and Y labels should be. The bacteria culture has a 2.3-hour doubling time. Starting concentration is 3.3x10^3 cells/ml. There has been 20 generation since the start of growth. Give an approximate time when the culture will reach the stationary phase taking into account all phases of the growth curve. (Calculation Spt, Graph with labels 5 pt)
Below is a figure of a typical bacterial growth curve. Use this to answer the questions below. Log Cell number 6. What stage is labeled A? 7. Name two things that are happening at the genetic and/or molecular level in the cell at this stage if cells in stationary phase were transferred from a media containing glucose to one containing lactose. 8. If the culture used to generate the growth curve above was started with 300 bacterial cells (generation 0),...
1. What is NOT a useful approach to generate a tumor environment most like that in situ? Co-culture tumor cells with other regional cell types Develop a 3D culture system Use a defined medium (that is, without serum) Vary glucose concentration over time to mimic in situ metabolism
2. What mode of reproduction do bacteria use? Describe a bacterial growth curve. Describe what happens at each step along the curve. What are some parameters that would affect how the curve looks? How would you predict the curve would look under different conditions? 17. Big Red's spot test produced the results shown below: What conclusions can be drawn from these results?
a)predict using graph what the potential energy, kinetic energy and total energy look like for a ball dropped from rest b)predict using graph what the potential energy, kinetic energy and total energy look like for a ball that is dropped from rest and bounce three times
Draw what the results for a Northern blot, probing for ara a mRNA, would look like for E. coli cells grown under the following conditions: 1) arabinose absent and glucose present, 2) arabinose present and glucose absent, 3) arabinose present and glucose present. Explain your reasoning.