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Assume that the growth curve was done using complex medium like TSB. Predict what the growth...


Assume that the growth curve was done using complex medium like TSB. Predict what the growth curve would look like if the culture was grown in a defined, minmal medium (containing glucose).
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Answer #1

In a minimal medium different amino acids remain absent hence the growth rate will be slow as compared to TSB. In presence of glucose organism will take time to produce enzymes due to Glucose Effect. So initially growth will be slow and after the production of enzymes growth will be faster. In a minimal media with glucose organism will show diauxic shift. The growth curve will be similar to classic curve but adaptation time or lag phase will be longer than in TSB.

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