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1.   What is NOT a useful approach to generate a tumor environment most like that in...

1.   What is NOT a useful approach to generate a tumor environment most like that in situ?

  1. Co-culture tumor cells with other regional cell types
  2. Develop a 3D culture system
  3. Use a defined medium (that is, without serum)
  4. Vary glucose concentration over time to mimic in situ metabolism
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Answer #1

Correct answer is C.

it is not useful to use a defined medium (that is, without serum) to generate a tumor environment most like that in situ because concentration of cell medium is not fixed, i.e. of glucose. It keeps on changing with time and also it includes interaction with other proteins, enzymes and metabolites.

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