Question

5) p points) Temperature regulation is critical while preforming PCR. What could resuit IT the PCR reaction is not cooled to 42°C -65°C before the extension step? Why would this occur (2 points) If you start with one copy of a single gene of interest and perform PCR on that sequence, how many copies of the gene would you have after 10 cycles? Explain your reasoning.

Answer 1

I am submitting your answer in image please look into it.

Thanks

? If PCR Reaction is not cooled before extension Step Mismatch hybrid are formed, resulting false product • Annealing temperature must be low enough to allow both forward and givesse primer to bind to the template, but high enough to prevent forming Mismatched hybrid • Both very low and very bligh temp. Can affect Beaction efficieney. This ideal temp. can be determining by caleulating melting temp. (Tm) -) By a simple formulę. Im = (4x[G+c]) + (2xCATT)) ° ③ PCR is a invitro method for amplify the target DNA. • Number of copies in a cycle of per calculate by a simple formulę !. Where na No. of Cylle. Here we have to cycle So n=10 put into formule. - 20 = 1024 Answer: we can get 1024 copies of the gene after to cycle of per.