One of these statements is incorrect. Which one is it? Please explain. Are the primers in...
One of these statements is incorrect. Which one is it? Please explain.
Are the primers in Polymerase Chain Reaction a starting place for replication?
Does PCR provide the information on abundance of specific gene targets?
Does PCR require a specific DNA Polymerase that is heat resistant?
Homework Answers
No PCR does not provide the any information on the abundance of the specific gene targets. In PCR we use the Total isolated DNA and use the specific primer for gene amplification.
Add Answer to:
One of these statements is incorrect. Which one is it? Please
explain.
Are the primers in...
A protein binds a DNA sequence downstream of a promoter. This results in an increased rate...
A protein binds a DNA sequence downstream of a promoter. This results in an increased rate of transcription of the gene. Which of the following site is likely to be attached? a. Operon b. Terminator c. Activator binding site d. Promotor The following are produced by algae To prepare the insert for the gene cloning, you need to find a proper source of the DNA fragment. PCR is one of the most common ways to get the gene that you...
EXPLAIN each answer thoroughly. There are two common goals when using PCR to amplify DNA. A....
EXPLAIN each answer thoroughly. There are two common goals when using PCR to amplify DNA. A. Make lots of copies of a specific DNA sequence to use in cloning (preparative PCR) B. Detect the presence or relative amount of a specific gene under varying conditions (analytical PCR) *Remember: PCR is very similiar to DNA replication, but uses a DNA polymerase to amplify only specific parts of DNA sequence based on the sequence of the primers. 3. For which goal (A...
*Microbiology. Please answer the below question. The current selected answer is incorrect. Thank you! Incorrect 0/1...
*Microbiology. Please answer the below question. The current
selected answer is incorrect. Thank you!
Incorrect 0/1 pts Question 1 Which of the following PCR components serves as the template? a. Target gene sequence • b. Synthetic primers c. Deoxyribonucleotides d. DNA polymerase III
Please answer the following questions about primers: a) Explain how the primers are made for the...
Please answer the following questions about primers: a) Explain how the primers are made for the Sanger sequencing when we even don't know the sequence of the DNA yet ? b) Do all cells in the body have the same primer and starting site for the DNA polymerase ? Do all primers have an idetical base sequence ? Is the same set of primers used for both leading and lagging strands ? c) Are primers made of DNA or RNA...
Which of the following statements about DNA replication is FALSE? Primase synthesizes the primers. DNA polymerase...
Which of the following statements about DNA replication is FALSE? Primase synthesizes the primers. DNA polymerase is required to add new nucleotides to the growing ends of the DNA strands. DNA ligase joins the small DNA fragments of the lagging strand. Only one strand of the parent DNA serves as a template for a newly synthesized complementary strand.
Carolina Savirana Craz 3/12/20 GECC-Polymerase Chain Reaction 1. What is the purpose of the polymerase chain...
Carolina Savirana Craz 3/12/20 GECC-Polymerase Chain Reaction 1. What is the purpose of the polymerase chain reaction? a. To repair damaged DNA b. To make copies of entire chromosomes c. To make copies of specific regions of DNA d. To prepare cells for cell division 2. The polymerase chain reaction is most comparable to what cellular process? a. Mitosis b. Replication c. Transcription d. Translation 3. When enzymes are elongating (building) a newly synthesized DNA strand in PCR, new nucleotides...
Which is not one of the elements needed to amplify DNA by polymerase chain reaction (PCR)?...
Which is not one of the elements needed to amplify DNA by polymerase chain reaction (PCR)? Question 16 options: A) Nucleoside triphosphates that serve as the source of the nucleotides A, T, C, and G needed in the synthesis of the new strands of DNA B) A restriction endonuclease enzyme that cleaves DNA at specific locations C) The segment of DNA that must be copied D) A DNA polymerase enzyme that will catalyze the synthesis of a complementary strand of...
12. To see if your polymerase chain reaction was successful and it amplified the right sequence...
12. To see if your polymerase chain reaction was successful and it amplified the right sequence of interest, you electrophorese the products from the reaction on an agarose gel. After staining the gel you find there are two bands of different sizes. Which one of the following is a possible explanation for these results? (1 point) a. The PCR induced a frame shift mutation into the DNA sequence. b. RNA polymerase copied the DNA into two copies of RNA. c....
Now. you should be able to answer the following questions: • How the amplification will be...
Now. you should be able to answer the following questions: • How the amplification will be done? - How you will determine your target sequence? How the amplification will be specific for certain segment? What are the requirements to carry PCR? • Suppose you perform a PCR that begins with one double-strand of the following DNA template: +5'-CTACCTGCGGGTTGACTGCTACCTTCCCGGGATGCCCAAAATTCTCGAG-3+ +3'-GATGGACGCCCAACTGACGATGGAAGGGCCCTACGGGTTTTAAGAGCTC-5'+ A. Draw one cycle of PCR reaction below the following diagram. B. Label the template DNA, the primers, and what is...
The direct role of the DNA denaturation step in a PCR reaction is to Group of...
The direct role of the DNA denaturation step in a PCR reaction is to Group of answer choices provide the optimal temperature for the DNA polymerase used separate the two DNA strands of the template allow the primers to anneal to the DNA 2. Which of the following would be useful/necessary for an expression vector BUT not for a cloning vector? Group of answer choices origin of replication regulatory sequences antibiotic resistance gene high copy number
*Microbiology. Please answer the below question. The current
selected answer is incorrect. Thank you!
Incorrect 0/1 pts Question 1 Which of the following PCR components serves as the template? a. Target gene sequence • b. Synthetic primers c. Deoxyribonucleotides d. DNA polymerase III
Carolina Savirana Craz 3/12/20 GECC-Polymerase Chain Reaction 1. What is the purpose of the polymerase chain reaction? a. To repair damaged DNA b. To make copies of entire chromosomes c. To make copies of specific regions of DNA d. To prepare cells for cell division 2. The polymerase chain reaction is most comparable to what cellular process? a. Mitosis b. Replication c. Transcription d. Translation 3. When enzymes are elongating (building) a newly synthesized DNA strand in PCR, new nucleotides...
Now. you should be able to answer the following questions: • How the amplification will be done? - How you will determine your target sequence? How the amplification will be specific for certain segment? What are the requirements to carry PCR? • Suppose you perform a PCR that begins with one double-strand of the following DNA template: +5'-CTACCTGCGGGTTGACTGCTACCTTCCCGGGATGCCCAAAATTCTCGAG-3+ +3'-GATGGACGCCCAACTGACGATGGAAGGGCCCTACGGGTTTTAAGAGCTC-5'+ A. Draw one cycle of PCR reaction below the following diagram. B. Label the template DNA, the primers, and what is...
Most questions answered within 3 hours.
-
A speeding car is traveling along a straight road with a
constant speed of 100 km/hour....
asked 48 seconds ago -
When 60.1 gg of calcium is reacted with nitrogen gas, 31.7 gg of
calcium nitride is...
asked 5 minutes ago -
The random variable X is exponentially distributed, where X
represents the time it takes for a...
asked 20 minutes ago -
a) Write a verilog module for 1:4 Demultiplexer using verilog
primitives.
b) Design 1-to-4 DEMUX using...
asked 15 minutes ago -
MATLAB
write a MATLAB function (1) output a third-order polynomial
function with the coefficients as the...
asked 27 minutes ago -
A z-score
communicates a raw score’s "relative standing"
under the normal curve in relation to:
asked 36 minutes ago -
An object is vibrating on a spring with the following equation
of motion:
?=(30 ??)cos((2?)/(160)?)
a)...
asked 35 minutes ago -
Vulcan Flyovers offers scenic overflights of Mount St. Helens,
the volcano in Washington State that explosively...
asked 38 minutes ago -
If organizations do not adapt fast enough and move incrementally
from the twentieth-century model to the...
asked 39 minutes ago -
A helium balloon with 2.5L of gas has a gauge pressure of 10,000
Pa. The balloon...
asked 43 minutes ago -
What is responsible for Jupiter's enormous magnetic field?
asked 58 minutes ago -
At the end of the year, a company offered to buy 5,000 units of
a product...
asked 1 hour ago