Question

D Question 12 3 pts Intact mitochondria fully capable of B-oxidation are incubated with palmitic acid and tritiated water, H20. Tritium, PH, is a radioactive isotope of hydrogen. You isolate tritiated acetate containing two PH atoms at C-2. PH2CH-COO from the intact mitochondria. Assuming that acetate is only. produced from the palmitic acid, what two enzymes installed the PH atoms into the "H2CH-COO? ATP synthase and thiolase A3, A2-enoyl-CoA isomerase and carnitine acyltransferase Thiolase and enoyl-CoA hydratase Acyl-CoA dehydrogenase and B-hydroacyl-CoA dehydrogenase 2,4-Dienoyl-CoA reductase and enoyl-CoA isomerase

Answer 1

Ans is enoyl CoA hydratase and thiolase.

Reason: enoyl CoA adds -OH on beta carbon and -H3 on alpha carbon.

Thiolase is acetyl coenzyme A acetyl tranferases which transfer CoASH on beta carbon and H3 on alpha carbon.