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1. It is essential for oxidative phosphorylation that the inner mitochondrial membrane remains intact. What happens if there
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1. It is very important that during oxidative phosphorylation the inner mitochondrial membrane remains intact. If there will be gaps in between the membrane, then there will be leakage of protons. During electron transport chain, when electrons are transported from one electron carrier to the other, protons are also pumped from mitochondrial Matrix to intermembrane space. If there is gap between inner mitochondrial membrane, then Proton will leak. This will disrupt the whole Proton gradient. And then there will be no generation of ATP. The electrochemical energy stored in Proton gradient is used by ATP synthase enzyme for the synthesis of ATP.

2. If the polarity of electron transport proteins is randomised, then oxidative phosphorylation will not take place. Electron transfer proteins are located on the membrane in such a way that they always transfer electron from one electron carrier to the other, all of which are arranged in increasing order of their electrode potential. They are arranged so because the highest electrode potential is that of oxygen which finally accepts the electron to form water. Also the electron Carriers are arranged so that they always pump protons from mitochondrial Matrix into intermembrane space to create a Proton gradient. If the polarity of electron transport protein is randomised, then Water will not be formed and because of destruction of proton gradient ATP will not also be formed.

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