Question

please help with genetics !!

5. Translate the RNA sequence below using three possible reading frames. 5' AGUCUAGGCACUGA 3' If this segment of RNA is found in the middle of an mRNA for a large protein, would you know which reading frame was used? How? 6. For each of the following sequences, rank them in order as sequences that could be used for initiation in eukaryotes. 1=best, 4=worst 5' GACGCCAUGG 3' 5' GCCUCCAUGC 3' 5' GCCAUCAAGG 3' 5' GCCACCAUGG 3'

Answer 1

5) for a single-stranded RNA there are three possible reading frames one starts at the first nucleotide at the 5` end, the second reading frame starts at the second nucleotide at the 5` end, the third reading frame starts at the third nucleotide from the 5` end.

the amino acid sequence using the first reading frame

Ser-Leu-Gly-Thr

the amino acid sequence using the second reading frame

Val-Ala-Leu

the amino acid sequence using the third reading frame

Ser-Arg-His-

so the given sequence is part of an mRNA for a large protein because the given mRNA does not have an open reading frame, so the first reading frame is used, in the original mRNA it does not have any stop codon in between, the stop codon is represented by `-` the other two reading frames have stop codons in between.