Question

3. Translation. According to the rules of the genetic code, there are six different reading frames in a double- stranded DNA molecule. One DNA strand serves as a template for transcription, which is complementary and antiparallel to the RNA product. The other DNA strand is the coding strand, which is identical in sequence to the RNA except for the substitution of uracil for thymine bases. A) There is a single open-reading frame (ORF) in the DNA molecule shown below. [Recall that an ORF begins with a start codon and ends with a stop codon.] Find the ORF in one of the DNA strands. Draw a box around the ORF in its coding strand 5-CGATCGCTATAAGGTTGACCTAGAGTTCGATTTACTCGTTTATGTGGCAGGCCATTTCTAAA-3 3-GCTAGCGATATTCCAACTGGACTCAAGCTAAATGAGCAAATACACCGTCCGGTAAAGATTT- B) Translate the ORF into a protein sequence. In the space provided below, write the protein's sequence. Use single-letter abbreviations for the amino acid residues. ] (C-terminus) (N-terminus)-

Answer 1

I think there is a problem with the sequence since the start codon is AUG that is coded by 5'-ATG-3' which is not to be seen in the sequence. Hence although it says that there is a single orf available but i dont think its there by mistake. So no ORF, No protein sequence either.

I have verified this by running the sequence in ORF finder as well, it also shows that there is no ORF in this sequence.

If you have any further doubt, please leave a comment down below. Thank you.