Question

yule grapn below comparing two different enzymes with different enzyme kinetics. a. Do the graphs have any of the same values for their Vmax, Vmax/2 or Km? If so which are the affinity for substrate same which are different? Enzyme with high Enzyme with low affinity for substrate Difference in reaction rate at low [5] Reaction rater Low (S] Substrate concentration [5] b. READ THIS: Enzyme-substrate affinity is the tendency for enzyme and substrate to bind together and form the enzyme substrate complex. Enzymes with high affinity for their substrate will readily form the Enzyme Substrate complex. Therefore they will need a low substrate concentration to reach Vmax/2 and will have a low Km. Enzymes with a low affinity for the substrate will not readily form the enzyme substrate complex. Therefore they will need a high substrate concentration to reach Vmax/2 and will have a high Km. It is convenient to use km as a measure of the affinity between the enzyme and substrate: this is an inverse relationship. Fill it in below: LOW Affinity - __ _Km High Affinity _ Km.

Answer 1

	Vmax	Vmax/2	Km
Enzyme with high affinity for substrate	different	different	same
Enzyme with low affinity for substrate	different	different	same

Both the enzymes are having a same value for Km. Km is the substrate concentration at which the velocity of an enzyme catalysed reaction is half of maximum velocity.

The more efficient enzyme is the one which is having a lower value of Km because it is requiring less amount of substrate to reach half of maximum velocity. But when the value of Km for both the enzymes is same, then we will compare the maximum velocity or half of maximum velocity for those enzymes. The one having more value of maximum velocity will be more efficient.

So the enzyme with high affinity for substrate is having more value for maximum velocity and therefore will be much more efficient than that of the enzyme having low affinity for substrate.

Low affinity = high Km

High affinity = low Km

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