Question

7. List the components (ingredients) required for a PCR reaction. 8. List the three main steps of a PCR reaction in order, from start to finish, of one eyele. 9. In addition to the components required for PCR, what is additionally required for Sanger sequencing, and why (what does it do)? | 10. Approximately how 'big' was the piece of DNA that we amplified? 11. Our DNA samples were quantified using a fluorometer. When we received the quantification results, what units of concentration were they in?

Answer 1

7.a) template

b) mgcl2

C) buffer

D)forward primer

E) backward primer

F) dNTPS G) Distilled water

8.step1:Denaturation

Step2: Annealing

Step3: extension

9.In sangers method, ddNTPs are used additional to the PCR master mix. This method is used for DNA sequencing and during primer extension, the random insertion of a ddNTPs instead of dNTPs terminates synthesis of chain.

10.Most of the PCR methods can amplify DNA fragments of between 0.1 to 10 kbp in length.

11.fluorometer can use UV, blue and LED rays and can analysis 1-2ul of sample only.