Question

a. List the six main components of a PCR amplification reaction and explain how its preseneel master mix is need for the production of an amplicon. Components Template DNA Primers MgCl2 dNTP Buffer Polymerase Why it is needed on

Answer 1

TEMPLATE DNA:	the strand used by DNA-taq-polymerase to attach complementary bases during DNA replication.
PRIMER:	PCR primers are short fragments of single stranded DNA usually 15-30 nucleotides in length. This is complementary to DNA sequences that flank the target region of interest. PCR primers provide a free 3’- OH group to which the DNA taq polymerase can add dNTPs.
MgCl2:	MgCl2 acts as a cofactor and is a catalyzer in PCR. At high concentrations of MgCl2 increases productivity of Taq polymerase.
Deoxynucleotide triphosphates (dNTPs):	These bases supply to the polymerase to make DNA strand.
Buffer:	PCR buffer is crucial to create optimal conditions for Taq DNA polymerase activity. Buffers contains Tris-Hcl, KCl and MgCl2.
Polymerase:	it synthesizes new DNA strands which are complementary to the target sequence. Usually used DNA Polymerase is taq DNA polymerase.