Homework Answers
8.
Components used in PCR
a. DNA template(to be copied)
b. Set of primers(forward and reverse primers)
c. dNTP
d. MgCl2
e. Taq polymerase
f. Taq polymerase buffer
g. Water
The components used in qPCR
a. template DNA(to be copied)
b. Set of primers(forward and reverse primers)
c. water
d. dNTP
e. MgCl2
f. Taq polymerase
g. Taq polymerase buffer
h. Fluorescent oligonucleotide probe
1. The correct option is- B
Primers designed should always be complementary to the
respective template DNA.
Primers designed should be at least 18-22 bp long and the GC
content should be in and around 40-60%.
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QUESTION 8 List the components used in PCR versus the components used in qPCR. QUESTION 1...
The DNA primers used in PCR are a. complementary to DNA sequences at both ends of...
The DNA primers used in PCR are a. complementary to DNA sequences at both ends of the DNA sequence of interest. b. attached to the gene of interest by ligase. c. produced when a gene of interest is read by restriction enzymes. d. identical to the entire base sequence of one strand of the DNA.
3’-TATAAAGACTTACAAATTTGTCCCCATTTTGC-5’ 5’-ATATTTCTGAATGTTTAAACAGGGGTAAAACG-3’ a. Diagram the results you would obtain for 1 and 2 rounds of PCR replication using the primers, 5’-ATGTT-3’ and 3’-CCATT-...
3’-TATAAAGACTTACAAATTTGTCCCCATTTTGC-5’ 5’-ATATTTCTGAATGTTTAAACAGGGGTAAAACG-3’ a. Diagram the results you would obtain for 1 and 2 rounds of PCR replication using the primers, 5’-ATGTT-3’ and 3’-CCATT-5’ and template above. b. The primers in part b were designed to make it easy to illustrate the PCR process, in practice PCR primers are 18-25bp in length. Why do PCR primers that are 5bp fail? c. Are primers used in PCR RNA or DNA? d. Explain how PCR amplifies the gene of interest without amplifying the...
1) You have two human liver cells (A and B) and you hypothesize that the insulin...
1) You have two human liver cells (A and B) and you hypothesize that the insulin receptor gene in Cell A has a mutation in exon 1 and Cell B contains the wild type sequence. You extract genomic DNA from each of the cells. Of the following, what would be the most efficient (quick, precise and relatively cheap) way to test your hypothesis. a. Isolate protein from both cells, purify the insulin receptor, and determine the amino acid content. b. Sequence the...
QUESTION 1: You are inserting a gene into an MCS found within the LacZ gene. Using...
QUESTION 1: You are inserting a gene into an MCS found within the LacZ gene. Using blue/white colony selection, why could you assume that white colonies have modified plasmids? a. A blue colony means the LacZ reading-frame was disrupted b. A blue colony means your gene has mutations c. A white colony means the LacZ reading-frame is intact d. A white colony means the LacZ reading-frame was disrupted QUESTION 2: You are performing a PCR using primers with a sequence perfectly...
*Microbiology. Please answer the below question. The current selected answer is incorrect. Thank you! Incorrect 0/1...
*Microbiology. Please answer the below question. The current
selected answer is incorrect. Thank you!
Incorrect 0/1 pts Question 1 Which of the following PCR components serves as the template? a. Target gene sequence • b. Synthetic primers c. Deoxyribonucleotides d. DNA polymerase III
8. PCR is used to. A Diagnose genetic disease 8 Solve cnmes C Sudy gene unction...
8. PCR is used to. A Diagnose genetic disease 8 Solve cnmes C Sudy gene unction D. All of th C ONA as a template to form RINA D All of the above 7. PCR technique does not need A. Tag polymerase B Restriion encymes C Olgoucletide prmers C. A fragment of skin D. All of the above 9 PCR can be used in A Cloning B.Sequening C.Medical dagnosis&foric mine 0.PCR can make mullple copies ot A. DNA B RNA...
NEED HELP WITH THESE QUESTIONS. PLEASE ANSWER ALL AND EXPLAIN AS WELL. THANKSSSSSSS 1. You want...
NEED HELP WITH THESE QUESTIONS. PLEASE ANSWER ALL AND EXPLAIN AS
WELL. THANKSSSSSSS
1. You want to clone a gene from a donor vector to a host vector. List the correct order of events in the process of cloning a. Perform ligation reaction of cloned gene and host vector. b. Perform double digestion of both donor and host vectors with the 2 restriction enzymes c. Examine donor and host vectors for restriction sites d. Purify cloned gene from donor vector...
1.When cloning a PCR product into a plasmid using restriction enzymes, the restriction enzyme recognition sequences...
1.When cloning a PCR product into a plasmid using restriction enzymes, the restriction enzyme recognition sequences in the PCR product most likely came from _______, and the restriction enzyme recognition sequences in the plasmid most likely came from ________. a. A multiple cloning site / the primers b. The primers / a multiple cloning site c. Both came from primers d. Both came from the multiple cloning site e. Naturally present in the gene of interest / the multiple cloning...
Question 1: Saved Advantages of the Polymerase Chain Reaction include all of these, except: 1) The...
Question 1: Saved Advantages of the Polymerase Chain Reaction include all of these, except: 1) The reaction is specific for certain sequences in the DNA. 2) Only small amounts of template are needed. 3) Results can only be obtained with freshly isolated DNA. 4) All the products from a specific part of the DNA will be the same size. Question 2: Saving The chain terminator used in Sanger's DNA sequencing is works due to.. 1) lack of 2'OH 2) ddNTP:...
solve for gel preparation and PCR mastermix calculations with steps for understanding TBE Buffer Calculations Determine...
solve for gel preparation and PCR mastermix calculations with
steps for understanding
TBE Buffer Calculations Determine the mass of the following reagents for a 10X stock 700mM of Tris Base (157g/mol) 887mM of Boric Acid (62g/mol) 25.7mM of EDTA (292g/mol) Dissolve in 750ml of DIH.O and bring to volume (IL) Calculate the dilution of your 10x stock for a 1X working stock. Remember you only need IL of working stock for a single experiment. Gel Preparation Calculations You need to...
*Microbiology. Please answer the below question. The current
selected answer is incorrect. Thank you!
Incorrect 0/1 pts Question 1 Which of the following PCR components serves as the template? a. Target gene sequence • b. Synthetic primers c. Deoxyribonucleotides d. DNA polymerase III
8. PCR is used to. A Diagnose genetic disease 8 Solve cnmes C Sudy gene unction D. All of th C ONA as a template to form RINA D All of the above 7. PCR technique does not need A. Tag polymerase B Restriion encymes C Olgoucletide prmers C. A fragment of skin D. All of the above 9 PCR can be used in A Cloning B.Sequening C.Medical dagnosis&foric mine 0.PCR can make mullple copies ot A. DNA B RNA...
NEED HELP WITH THESE QUESTIONS. PLEASE ANSWER ALL AND EXPLAIN AS
WELL. THANKSSSSSSS
1. You want to clone a gene from a donor vector to a host vector. List the correct order of events in the process of cloning a. Perform ligation reaction of cloned gene and host vector. b. Perform double digestion of both donor and host vectors with the 2 restriction enzymes c. Examine donor and host vectors for restriction sites d. Purify cloned gene from donor vector...
solve for gel preparation and PCR mastermix calculations with
steps for understanding
TBE Buffer Calculations Determine the mass of the following reagents for a 10X stock 700mM of Tris Base (157g/mol) 887mM of Boric Acid (62g/mol) 25.7mM of EDTA (292g/mol) Dissolve in 750ml of DIH.O and bring to volume (IL) Calculate the dilution of your 10x stock for a 1X working stock. Remember you only need IL of working stock for a single experiment. Gel Preparation Calculations You need to...
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