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Gel electrophoresis is used to separate DNA fragments according to their size and samples are loaded at one end of the gel and an electric current is applied through ger then DNA is negatively charged thats why they move towards the positive electrode. 3) Ansi chelax is used in DNA extraction in preparation of polymerase chain reaction and polar resin beads bind polar cellular components after breaking open cells - 47 Ansi- DNA is important for inheritance, - coding for proteons. It is vital for all t'ving organisen from DNA newborn genetic disease can be determined and analyze forensic ev volence DNA involve in an organisons cell development and reprodvetion.
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1. List the ingredients required for PCR. 2. How does DNA move down the agarose gel?...
7. List the components (ingredients) required for a PCR reaction. 8. List the three main steps...
7. List the components (ingredients) required for a PCR reaction. 8. List the three main steps of a PCR reaction in order, from start to finish, of one eyele. 9. In addition to the components required for PCR, what is additionally required for Sanger sequencing, and why (what does it do)? | 10. Approximately how 'big' was the piece of DNA that we amplified? 11. Our DNA samples were quantified using a fluorometer. When we received the quantification results, what...
1. Explain the difference between an intron and an exon. 2. Why do the two possible...
1. Explain the difference between an intron and an exon. 2. Why do the two possible PCR products differ in size by 300 base pairs? 3. Explain how agarose electrophoresis separates DNA fragments. Why does a smaller DNA fragment move faster than a larger one? 4. What kind of controls are run in this experiment? Why are they important? Could others be used?
14. Like a ladder. Circular DNA from the SV40 virus was isolated and subjected to gel...
14. Like a ladder. Circular DNA from the SV40 virus was isolated and subjected to gel electrophoresis. The results are shown in lane A (the control) of the adjoining gel patterns. (a) Why does the DNA separate in agarose gel electrophoresis? How does the DNA in each band differ? (b) What types of DNA do the various bands represent? (c) What is the significance of the fact that more of the DNA is in slower-moving forms?
1. What is the purpose of the PCR nucleotide mix added to your PCR reaction? 2....
1. What is the purpose of the PCR nucleotide mix added to your PCR reaction? 2. What is the purpose of Gel Green? What type of chemical is it? Molecularly how does it work? 3. Why are the restriction digest reactions mixed by flicking or pipetting, rather than vortexing? 4. In the ligation, hAPP can only insert in one orientation into the vector why is this? What is the role of hAPP in DNA replication? What is its role in...
1. what are the differences between DNA replication vs. PCR? 2. From gel electrolysis, what does...
1. what are the differences between DNA replication vs. PCR? 2. From gel electrolysis, what does the DNA strand look like? 3. Why is cancer a genetic disease but are not inherited?
FULL image displayed under of the expected vs actual results of agarose gel analysis (the top...
FULL image displayed under of the expected vs actual results of
agarose gel analysis (the top picture is expected and bottom is the
result)
Question: You conclude that the error occurred while preparing
arose gel. What mistake was made? Why does it look like the picture
above and not how you expected?
Gel Question #1: During Lab 7, you and your three teammates performed gel electrophoresis as described in the lab manual. Your team was especially careful while carrying out...
After PCR is performed the products are run out on an agarose gel. In the figure...
After PCR is performed the products are run out on an agarose
gel. In the figure below, grey bands represent the wells the PCR
product was loaded into. The white bands represent DNA fragments
produced by PCR. The target fragment amplified by the primers was
1,500 bp in size.
The ladder is a standard DNA ladder containing bands of various
sizes between 5,000 and 1,000 bp. The negative control contained
only molecular grade water*. The positive control contained DNA
known...
Gel Electrophoresis of Amplified PCR Samples 9. What is Alu? 10. Why is Alu useful for...
Gel Electrophoresis of Amplified PCR Samples 9. What is Alu? 10. Why is Alu useful for studying human ancestry? 11. Why do the two possible PCR products from our lab differ in size by 300 base pairs? 12. Explain how gel electrophoresis separates DNA fragments 13. Fill in the table below. For each genotype, write how many DNA bands (fragments) you would expect to see in a gel, along with the size of each (n base pairs) Table 1. Predicted...
The PCR procedure can be performed with fluorescently labeled primers and polyacrylamide gel electrophoresis (PAGE). What...
The PCR procedure can be performed with fluorescently labeled
primers and polyacrylamide gel electrophoresis (PAGE). What would
be the advantage of this procedure over agarose gel analysis?
PAGE allows for higher resolution than the agarose gel
procedure.
The PAGE procedure is faster than that using agarose gel
electrophoresis.
The labeled primers are less expensive because they are used at
a lower concentration in the reaction mix.
The PAGE procedure is less prone to contamination
Case Study 12-2 A 7-month-old child...
Gel Electrophoresis and Bioinformatics Analyses of wild type and mutant Arabidopsis 1. The procedure for making...
Gel Electrophoresis and Bioinformatics Analyses of wild type and mutant Arabidopsis 1. The procedure for making 30 mL of 1.2% agarose in 1x TAE is: g agarose mL lX TAE 2. Briefly explain the roles of each of the following in electrophoresis. Agarose - Tris - EDTA- Power supply - 3. What is the stain we used to view DNA and how does it work? 4. Which samples amplified and which ones did not?
7. List the components (ingredients) required for a PCR reaction. 8. List the three main steps of a PCR reaction in order, from start to finish, of one eyele. 9. In addition to the components required for PCR, what is additionally required for Sanger sequencing, and why (what does it do)? | 10. Approximately how 'big' was the piece of DNA that we amplified? 11. Our DNA samples were quantified using a fluorometer. When we received the quantification results, what...
1. Explain the difference between an intron and an exon. 2. Why do the two possible PCR products differ in size by 300 base pairs? 3. Explain how agarose electrophoresis separates DNA fragments. Why does a smaller DNA fragment move faster than a larger one? 4. What kind of controls are run in this experiment? Why are they important? Could others be used?
FULL image displayed under of the expected vs actual results of
agarose gel analysis (the top picture is expected and bottom is the
result)
Question: You conclude that the error occurred while preparing
arose gel. What mistake was made? Why does it look like the picture
above and not how you expected?
Gel Question #1: During Lab 7, you and your three teammates performed gel electrophoresis as described in the lab manual. Your team was especially careful while carrying out...
After PCR is performed the products are run out on an agarose
gel. In the figure below, grey bands represent the wells the PCR
product was loaded into. The white bands represent DNA fragments
produced by PCR. The target fragment amplified by the primers was
1,500 bp in size.
The ladder is a standard DNA ladder containing bands of various
sizes between 5,000 and 1,000 bp. The negative control contained
only molecular grade water*. The positive control contained DNA
known...
Gel Electrophoresis of Amplified PCR Samples 9. What is Alu? 10. Why is Alu useful for studying human ancestry? 11. Why do the two possible PCR products from our lab differ in size by 300 base pairs? 12. Explain how gel electrophoresis separates DNA fragments 13. Fill in the table below. For each genotype, write how many DNA bands (fragments) you would expect to see in a gel, along with the size of each (n base pairs) Table 1. Predicted...
The PCR procedure can be performed with fluorescently labeled
primers and polyacrylamide gel electrophoresis (PAGE). What would
be the advantage of this procedure over agarose gel analysis?
PAGE allows for higher resolution than the agarose gel
procedure.
The PAGE procedure is faster than that using agarose gel
electrophoresis.
The labeled primers are less expensive because they are used at
a lower concentration in the reaction mix.
The PAGE procedure is less prone to contamination
Case Study 12-2 A 7-month-old child...
Gel Electrophoresis and Bioinformatics Analyses of wild type and mutant Arabidopsis 1. The procedure for making 30 mL of 1.2% agarose in 1x TAE is: g agarose mL lX TAE 2. Briefly explain the roles of each of the following in electrophoresis. Agarose - Tris - EDTA- Power supply - 3. What is the stain we used to view DNA and how does it work? 4. Which samples amplified and which ones did not?
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