The three main platforms to generate high-throughput sequence data are Illumina (e.g., NovaSeq), Pacific Biosciences (PacBio), and Oxford Nanopore. Briefly describe their relative advantages/disadvantages?
Illumina dye sequence is used to calculate the group of base pairs in the DNA. This method is also known as DNA sequencing. This method is used for whole genome sequencing and as well as the region sequencing.
NovaSeq is a ground breaking discovery that objective is our proven technology.
Advantage : The immense stability and rang of application for the NovaSeq allow for effecient sequencing of whole animal , plant and human genome, whole exomes an large whole transcriptiome project at lowest per sample cost compair to the other illumina instruments.
Disadvantage : It is highly cost effective to the other illumina instruments ( NovaSeq 5000 and 6000 cost nearly $ 850000 and $ 985000 .
Pacific Biosciences : It is develops and manufactures system for gene sequencing and some novel real time biological observation. It is used for single molecule real time sequencing (SMRT), based on the properties of zero-mode waveguides.
Advantage : It has advantages like long read lengths,high consensus accuracy,a low degree of bias ,and simultaneous capability of epigenetic characterisation.
Disadvantage : Higher sequencing error rate .
The major problems is that the flow cell is not as high throughput when compared to the illumina sequence .
Oxford nanopore seq : It passes an ionic current through nanopores and measures the changes in current as biological molecules pass through the nanopores or near it .This information about change in voltage can be used to identify the molecule type .
Advantage : It is cheaper process as it requires minimal chemicals or enzymes than others.
Disadvantage : It has sequencing errors and can be as high as 15%. If sequencing large amounts of sample then the error rate can be tolerate by multiple copies of the same sequence which allow the user to recognise and eleminate mistakes or errors .
The three main platforms to generate high-throughput sequence data are Illumina (e.g., NovaSeq), Pacific Biosciences (PacBio),...
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