Question

cessive temperature produce inferior results in electrophoresis? Consider that the Objective is to produce a pattern in which the molecules of different sizes are well-resolved (well separated) Il you found that your mixture of large DNA fragments was poorly-resolved in a 0.8% agarose gel, would you switch to a higher or a lower percentage gel? Why? What is the purpose of omitting enzyme from tubes 1 and 3? What are the respective sizes (in bp) of the human and bacteriophage 1. genomes? Given that the recognition sequence for EcoRI is six base pairs, how often would you expect to find an EcoRI-site in a sequence of nucleotides? Once every ſhow many?) bp?

Answer 1

1.Excessive temperature results in high migration of DNA.The high migration results in poor resolution of DNA and smear type DNA

2.if large DNA fragments are poorley resolved on 0.8% gel ,you must use lower percentage gel.Because the sugars molecules that make up the agarose gel matrix act like sieve.The high agarose concentration makes the small pores in the gel,

3.i did not get what does it mean

4.human genome contain 3 billion basepairs and bacteriophage lambda contain 48,502 basepairs

5.Ecor1 restriction site occur every 4096 base pairs(4*4*4*4*4*4=4096)